Hubei Provincial Hospital of Traditional Chinese Medicine , Hubei Province Academy of Traditional Chinese Medicine , Wuhan 430061 , P. R. China.
Research Center for Micro/Nano System & Bionic Medicine, Institute of Biomedical & Health Engineering , Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences , Shenzhen 518055 , P. R. China.
Anal Chem. 2019 Dec 3;91(23):14838-14841. doi: 10.1021/acs.analchem.9b04422. Epub 2019 Nov 11.
We present a paper-based system that integrates bioluminescence resonance energy transfer (BRET) and isothermal amplification for the analysis of tumor-associated circulating microRNAs (miRNAs) in clinical serum samples. The analysis procedure could be easily accomplished with two pieces of functionalized paper and a low-cost smartphone-based device, which enables sequence-specific quantification of femtomolar miRNAs, without the need for tedious handling of aqueous reactions and operation of sophisticated equipment. Furthermore, the analytical performance of the proposed paper-based system was highly stable at room temperature, demonstrating its capability for cold-chain-free and remote deployment. These qualities highlight the practical utility of our method for the portable and field-ready miRNA diagnostic tests in resource-limited settings.
我们提出了一种基于纸张的系统，该系统将生物发光共振能量转移（BRET）和等温扩增集成在一起，用于分析临床血清样本中与肿瘤相关的循环 microRNAs（miRNAs）。该分析过程可以通过两块功能化纸张和低成本的基于智能手机的设备轻松完成，该设备能够实现纳摩尔级 miRNAs 的序列特异性定量，而无需繁琐的水溶液反应处理和复杂设备的操作。此外，所提出的基于纸张的系统在室温下具有高度稳定的分析性能，证明了其在无冷链和远程部署方面的能力。这些特性突出了我们的方法在资源有限环境下用于便携式和现场就绪的 miRNA 诊断测试的实际应用价值。
