College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Key Laboratory of Analytical Chemistry for Biology and Medicine, Wuhan 430072, Hubei Province, China.
Department of Radiology, Zhongnan Hospital of Wuhan University, Wuhan 430072, Hubei Province, China.
Anal Chem. 2024 Aug 13;96(32):13103-13109. doi: 10.1021/acs.analchem.4c01711. Epub 2024 Aug 4.
Due to invasive and serial examinations of bioactive molecules, liquid biopsy (LB) has emerged as a rapid and reliable solution for early disease detection and monitoring. Developing portable devices with high specificity and sensitivity for LB is highly valuable. To realize a generalized approach to increase the sensitivity of LB, we developed an ultrasensitive diagnostic biochip based on the amplification of miRNA by recombinase polymerase amplification and the significant enhancement of fluorescence signals by photonic crystal (PC) materials. The PCs-RPA biochip has a detection limit as low as 0.24 aM, a wide linear range of 8 orders of magnitude, and excellent specificity. Such advantages realize the accurate detection of circulating miRNAs with very low content in clinical serum samples for the precise diagnosis of nonsmall cell lung cancer.
由于对生物活性分子的侵入性和序列检查，液体活检 (LB) 已经成为早期疾病检测和监测的快速可靠的解决方案。开发具有高特异性和灵敏度的便携式 LB 设备具有很高的价值。为了实现提高 LB 灵敏度的通用方法，我们开发了一种基于重组酶聚合酶扩增放大 miRNA 和光子晶体 (PC) 材料显著增强荧光信号的超灵敏诊断生物芯片。该 PC-RPA 生物芯片的检测限低至 0.24 aM，线性范围宽达 8 个数量级，具有优异的特异性。这些优势实现了临床血清样本中含量非常低的循环 miRNA 的精确检测，从而实现了非小细胞肺癌的精确诊断。
