Yang Pan-Pan, Yu Xiao-Hui, Zhou Jiao
Department of Oncology, Jining First People's Hospital, Jining, P.R. China.
Department of Ophthalmology, Yantai Yuhuangding Hospital, Yantai, P.R. China.
Biosci Biotechnol Biochem. 2020 Mar;84(3):471-480. doi: 10.1080/09168451.2019.1686967. Epub 2019 Nov 6.
This study aimed to explore the influence of Tryptophanyl-tRNA synthetase (WARS) expression on the proliferation and migration of uveal melanoma (UM) cells, and the potential mechanisms. Bioinformatics analysis based on Gene Expression Omnibus (GEO) database showed that WARS expression in metastatic cancer was significantly higher than that in no-metastatic group. Kaplan-Meier analysis based on The Cancer Genome Atlas (TCGA) database showed that high WARS expression was associated with lower survival. Biological function experiments showed that overexpression of WARS in OCM-1A cells can promote cell proliferation, migration, and invasion, whereas knockdown of WARS in C918 cells showed the opposite effect. Finally, we observed that the up-regulation of WARS induced the activation of phosphatidylinositol 3-kinase/AKT (PI3K/AKT) signaling, whilst depletion of WARS resulted in opponent outcomes. Taken together, our results illustrated that WARS was overexpressed in UM cells and contributed to the viability and motility of UM cells via modulating PI3K/AKT signaling pathway.
本研究旨在探讨色氨酰 - tRNA合成酶（WARS）表达对葡萄膜黑色素瘤（UM）细胞增殖和迁移的影响及其潜在机制。基于基因表达综合数据库（GEO）的生物信息学分析表明，转移性癌症中WARS的表达明显高于非转移组。基于癌症基因组图谱（TCGA）数据库的Kaplan - Meier分析表明，WARS高表达与较低生存率相关。生物学功能实验表明，在OCM - 1A细胞中过表达WARS可促进细胞增殖、迁移和侵袭，而在C918细胞中敲低WARS则表现出相反的效果。最后，我们观察到WARS的上调诱导了磷脂酰肌醇3 - 激酶/蛋白激酶B（PI3K/AKT）信号通路的激活，而WARS的缺失则导致相反的结果。综上所述，我们的结果表明WARS在UM细胞中过表达，并通过调节PI3K/AKT信号通路促进UM细胞的活力和运动性。
