Fisheries College, Jimei University, Xiamen, 361021, China.
Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Xiamen, 361021, China.
BMC Plant Biol. 2019 Nov 6;19(1):475. doi: 10.1186/s12870-019-2076-4.
Pyropia haitanensis, distributes in the intertidal zone, can tolerate water losses exceeding 90%. However, the mechanisms enabling P. haitanensis to survive harsh conditions remain uncharacterized. To elucidate the mechanism underlying P. haitanensis desiccation tolerance, we completed an integrated analysis of its transcriptome and proteome as well as transgenic Chlamydomonas reinhardtii carrying a P. haitanensis gene.
P. haitanensis rapidly adjusted its physiological activities to compensate for water losses up to 60%, after which, photosynthesis, antioxidant systems, chaperones, and cytoskeleton were activated to response to severe desiccation stress. The integrative analysis suggested that transketolase (TKL) was affected by all desiccation treatments. Transgenic C. reinhardtii cells overexpressed PhTKL grew better than the wild-type cells in response to osmotic stress.
P. haitanensis quickly establishes acclimatory homeostasis regarding its transcriptome and proteome to ensure its thalli can recover after being rehydrated. Additionally, PhTKL is vital for P. haitanensis desiccation tolerance. The present data may provide new insights for the breeding of algae and plants exhibiting enhanced desiccation tolerance.
坛紫菜分布于潮间带，能耐受超过 90%的失水。然而，坛紫菜耐受恶劣条件的机制尚不清楚。为了阐明坛紫菜耐旱的机制，我们对其转录组和蛋白质组进行了综合分析，并对携带坛紫菜基因的转基因莱茵衣藻进行了分析。
坛紫菜在失水达 60%时能迅速调整其生理活动以进行补偿，之后，光合作用、抗氧化系统、伴侣蛋白和细胞骨架被激活以应对严重的干燥胁迫。综合分析表明，转酮醇酶（TKL）受到所有干燥处理的影响。过表达 PhTKL 的转基因莱茵衣藻细胞在应对渗透胁迫时比野生型细胞生长得更好。
坛紫菜迅速建立适应其转录组和蛋白质组的稳态以确保其藻体在再水合后能够恢复。此外，PhTKL 对坛紫菜的耐旱性至关重要。本研究结果可能为培育具有增强耐旱性的藻类和植物提供新的思路。
