Guan Zheng-Jun, Zheng Min, Tang Zhi-Xi, Wei Wei, Stewart C Neal
Department of Life Sciences, Yuncheng University, Yuncheng 044000, China.
State Key Laboratory of Vegetation and Climate Change, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
Plants (Basel). 2023 Jun 15;12(12):2319. doi: 10.3390/plants12122319.
Oilseed rape ( L.) is an important cash crop, but transgenic oilseed rape has not been grown on a commercial scale in China. It is necessary to analyze the characteristics of transgenic oilseed rape before commercial cultivation. In our study, differential expression of total protein from the leaves in two transgenic lines of oilseed rape expressing foreign Bt Cry1Ac insecticidal toxin and their non-transgenic parent plant was analyzed using a proteomic approach. Only shared changes in both of the two transgenic lines were calculated. Fourteen differential protein spots were analyzed and identified, namely, eleven upregulated expressed protein spots and three downregulated protein spots. These proteins are involved in photosynthesis, transporter function, metabolism, protein synthesis, and cell growth and differentiation. The changes of these protein spots in transgenic oilseed rape may be attributable to the insertion of the foreign transgenes. However, the transgenic manipulation might not necessarily cause significant change in proteomes of the oilseed rape.
油菜（L.）是一种重要的经济作物，但转基因油菜在中国尚未进行商业化种植。在商业化种植之前，有必要分析转基因油菜的特性。在我们的研究中，采用蛋白质组学方法分析了两个表达外源Bt Cry1Ac杀虫毒素的转基因油菜品系及其非转基因亲本植株叶片中总蛋白的差异表达。仅计算两个转基因品系共有的变化。分析并鉴定了14个差异蛋白点，即11个上调表达的蛋白点和3个下调表达的蛋白点。这些蛋白质参与光合作用、转运功能、代谢、蛋白质合成以及细胞生长和分化。转基因油菜中这些蛋白点的变化可能归因于外源转基因的插入。然而，转基因操作不一定会导致油菜蛋白质组发生显著变化。
