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人参皂苷Rg1通过诱导3T3-L1细胞和皮下白色脂肪细胞中的UCP1表达及线粒体活性来促进褐色化。

Ginsenoside Rg1 promotes browning by inducing UCP1 expression and mitochondrial activity in 3T3-L1 and subcutaneous white adipocytes.

作者信息

Lee Kippeum, Seo Young-Jin, Song Ji-Hyoen, Chei Sungwoo, Lee Boo-Yong

机构信息

Department of Food Science and Biotechnology, College of Life Science, CHA University, Kyeonggi, Republic of Korea.

出版信息

J Ginseng Res. 2019 Oct;43(4):589-599. doi: 10.1016/j.jgr.2018.07.005. Epub 2018 Jul 18.

DOI:10.1016/j.jgr.2018.07.005
PMID:31695565
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6823768/
Abstract

BACKGROUND

Meyer is known as a conventional herbal medicine, and ginsenoside Rg1, a steroid glycoside, is one of its components. Although Rg1 has been proved to have an antiobesity effect, the mechanism of this effect and whether it involves adipose browning have not been elucidated.

METHODS

3T3-L1 and subcutaneous white adipocytes from mice were used to access the thermogenic effect of Rg1. Adipose mitochondria and uncoupling protein 1 (UCP1) expression were analyzed by immunofluorescence. Protein level and mRNA of UCP1 were also evaluated by Western blotting and real-time polymerase chain reaction, respectively.

RESULTS

Rg1 dramatically enhanced expression of brown adipocyte-specific markers, such as UCP1 and fatty acid oxidation genes, including carnitine palmitoyltransferase 1. In addition, it modulated lipid metabolism, activated 5' adenosine monophosphate (AMP)-activated protein kinase, and promoted lipid droplet dispersion.

CONCLUSIONS

Rg1 increases UCP1 expression and mitochondrial biogenesis in 3T3-L1 and subcutaneous white adipose cells isolated from C57BL/6 mice. We suggest that Rg1 exerts its antiobesity effects by promoting adipocyte browning through activation of the AMP-activated protein kinase pathway.

摘要

背景

迈耶是一种传统草药，人参皂苷Rg1是一种甾体糖苷，是其成分之一。尽管Rg1已被证明具有抗肥胖作用，但其作用机制以及是否涉及脂肪褐变尚未阐明。

方法

使用3T3-L1细胞和小鼠皮下白色脂肪细胞来研究Rg1的产热作用。通过免疫荧光分析脂肪线粒体和解偶联蛋白1（UCP1）的表达。还分别通过蛋白质印迹法和实时聚合酶链反应评估UCP1的蛋白质水平和mRNA水平。

结果

Rg1显著增强了棕色脂肪细胞特异性标志物的表达，如UCP1和脂肪酸氧化基因，包括肉碱棕榈酰转移酶1。此外，它调节脂质代谢，激活5'-腺苷单磷酸（AMP）激活的蛋白激酶，并促进脂滴分散。

结论

Rg1增加了从C57BL/6小鼠分离的3T3-L1细胞和皮下白色脂肪细胞中UCP1的表达和线粒体生物发生。我们认为Rg1通过激活AMP激活的蛋白激酶途径促进脂肪细胞褐变来发挥其抗肥胖作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfb9/6823768/bc922e21312f/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfb9/6823768/014ca57d527a/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfb9/6823768/462bc59c5adc/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfb9/6823768/650aac4c3b1c/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfb9/6823768/72b4069bedee/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfb9/6823768/5668d1770841/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfb9/6823768/9941c8f76b5e/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfb9/6823768/b424651a9050/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfb9/6823768/bc922e21312f/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfb9/6823768/014ca57d527a/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfb9/6823768/462bc59c5adc/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfb9/6823768/650aac4c3b1c/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfb9/6823768/72b4069bedee/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfb9/6823768/5668d1770841/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfb9/6823768/9941c8f76b5e/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfb9/6823768/b424651a9050/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfb9/6823768/bc922e21312f/gr8.jpg

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