Department of Operation Room, The Affiliated Hospital of Qingdao University (Laoshan Branch Courts), Qingdao, China.
Eur Rev Med Pharmacol Sci. 2019 Oct;23(20):8984-8990. doi: 10.26355/eurrev_201910_19298.
MicroRNAs (miRNAs) have been demonstrated to have crucial roles in cancer development. We investigated the involvement of miR-223-3p in neuroblastoma (NB).
MiR-223-3p expression in NB cell lines and normal cell line was analyzed with real-time quantitative PCR method. Cell proliferation, cell invasion, and cell apoptosis were assessed by cell counting kit-8 (CCK-8), transwell invasion assay, and flow cytometry assay, respectively. Bioinformatics analysis, Dual-Luciferase reporter assays, and Western blot analysis were conducted to identify the connection of miR-223-3p and forkhead box O1 (FOXO1).
MiR-223-3p level was found highly expressed in NB cell lines compared with normal cell line. Knockdown miR-223-3p expression decreased cell growth and invasion but increased cell apoptosis. MiR-223-3p was able to bind with the 3'-untranslated region of FOXO1, and thereby resulting in a reduction of FOXO1 expression. The knockdown of FOXO1 increased the malignant capacity of NB cells.
Therefore, given the fact that miR-223-3p suppressed FOXO1 expression to promote NB progression, targeting miR-223-3p may be an effective method for NB treatment.
微小 RNA（miRNA）已被证明在癌症发展中具有重要作用。我们研究了 miR-223-3p 在神经母细胞瘤（NB）中的作用。
采用实时定量 PCR 方法分析 NB 细胞系和正常细胞系中 miR-223-3p 的表达。通过细胞计数试剂盒-8（CCK-8）、Transwell 侵袭实验和流式细胞术分别评估细胞增殖、细胞侵袭和细胞凋亡。通过生物信息学分析、双荧光素酶报告基因实验和 Western blot 分析确定 miR-223-3p 和叉头框蛋白 O1（FOXO1）的关系。
与正常细胞系相比，NB 细胞系中 miR-223-3p 水平表达较高。下调 miR-223-3p 表达可降低细胞生长和侵袭，但可增加细胞凋亡。miR-223-3p 能够与 FOXO1 的 3'-非翻译区结合，从而降低 FOXO1 的表达。FOXO1 的敲低增加了 NB 细胞的恶性能力。
因此，鉴于 miR-223-3p 通过抑制 FOXO1 表达促进 NB 进展的事实，靶向 miR-223-3p 可能是治疗 NB 的一种有效方法。
