Clinical Medicine, Cheeloo College of Medicine, Shandong University, Jinan, P. R. China.
Eur Rev Med Pharmacol Sci. 2019 Jan;23(2):716-723. doi: 10.26355/eurrev_201901_16885.
Dysregulation of microRNAs (miRNAs) expression often resulted in abnormal cell behaviors. It has been demonstrated that miRs may serve as oncogenic or tumor suppressive functions in tumor. We investigated whether or not miR-144-3p has a role in the progression of human neuroblastoma (NB).
46 NB patients were enrolled in this study. miR-144-3p expression in NB tissues and cell lines was detected by reverse transcription-quantitative polymerase chain reaction. The biological functions of miR-144-3p in NB were detected by cell counting kit-8 assay, flow cytometry assay, and wound-healing assay. Luciferase activity assay and Western blot assay were performed to validate the direct targets of miR-144-3p.
We found miR-144-3p expression was reduced in NB tissues and cell lines and resulted in the stimulation of cell proliferation, cell cycle progression, and cell migration in vitro. Furthermore, we validated homeobox protein A7 (HOXA7) as a direct target of miR-144-3p.
Taken together, these results demonstrated the tumor suppressive role of miR-144-3p in NB and may advance the understanding of the underlying mechanisms of miR-144-3p and HOXA7 in NB.
miRNAs 表达失调常导致细胞行为异常。研究表明，miRs 在肿瘤中可能具有致癌或抑癌功能。我们研究了 miR-144-3p 是否在人神经母细胞瘤（NB）的进展中起作用。
本研究纳入了 46 名 NB 患者。采用逆转录定量聚合酶链反应检测 NB 组织和细胞系中 miR-144-3p 的表达。通过细胞计数试剂盒-8 检测、流式细胞术检测和划痕愈合检测来检测 miR-144-3p 在 NB 中的生物学功能。采用荧光素酶活性检测和 Western blot 检测来验证 miR-144-3p 的直接靶标。
我们发现 miR-144-3p 在 NB 组织和细胞系中的表达降低，导致体外细胞增殖、细胞周期进程和细胞迁移的刺激。此外，我们验证了同源盒蛋白 A7（HOXA7）是 miR-144-3p 的直接靶标。
综上所述，这些结果表明 miR-144-3p 在 NB 中具有肿瘤抑制作用，并可能深入了解 miR-144-3p 和 HOXA7 在 NB 中的潜在机制。
