在使用MMC ImpRes混合模式色谱法纯化双特异性抗体过程中去除半抗体、孔-孔同二聚体和聚集体。
Removal of half antibody, hole-hole homodimer and aggregates during bispecific antibody purification using MMC ImpRes mixed-mode chromatography.
作者信息
Tang Jiaqin, Zhang Xudong, Chen Tao, Wang Ying, Li Yifeng
机构信息
Technology and Process Development (TPD), WuXi Biologics, 288 Fute Zhong Road, Waigaoqiao Free Trade Zone, Shanghai, 200131, China.
Technology and Process Development (TPD), WuXi Biologics, 288 Fute Zhong Road, Waigaoqiao Free Trade Zone, Shanghai, 200131, China.
出版信息
Protein Expr Purif. 2020 Mar;167:105529. doi: 10.1016/j.pep.2019.105529. Epub 2019 Nov 4.
During recombinant production of asymmetric IgG-like bispecific antibodies (bsAbs), various by-products are often observed due to unbalanced chain expression and incorrect chain pairing. Among them, half antibody and homodimer are found with high frequency. In this work, with a case study we demonstrated that Capto MMC ImpRes mixed-mode chromatography can effectively remove these two by-products as well as antibody aggregates under optimized conditions. This makes MMC ImpRes a powerful tool for bsAb purification.
在不对称IgG样双特异性抗体（bsAbs）的重组生产过程中，由于链表达不平衡和链配对不正确，经常会观察到各种副产物。其中，半抗体和同二聚体的出现频率较高。在这项工作中，通过一个案例研究，我们证明了在优化条件下，Capto MMC ImpRes混合模式色谱可以有效去除这两种副产物以及抗体聚集体。这使得MMC ImpRes成为bsAb纯化的有力工具。
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