Zhu Xiaoyu, Huo Shihan, Xue Chao, An Bo, Qu Jun
Department of Pharmaceutical Sciences, School of Pharmacy & Pharmaceutical Sciences, University at Buffalo, State University of New York, Buffalo, NY, 14214, USA.
New York State Center of Excellence in Bioinformatics & Life Sciences, Buffalo, NY, 14203, USA.
J Pharm Anal. 2020 Jun;10(3):209-220. doi: 10.1016/j.jpha.2020.05.008. Epub 2020 May 23.
The past few years have witnessed enormous progresses in the development of antibody-drug conjugates (ADCs). Consequently, comprehensive analysis of ADCs in biological systems is critical in supporting discovery, development and evaluation of these agents. Liquid chromatography-mass spectrometry (LC-MS) has emerged as a promising and versatile tool for ADC analysis across a wide range of scenarios, owing to its multiplexing ability, rapid method development, as well as the capability of analyzing a variety of targets ranging from small-molecule payloads to the intact protein with a high, molecular resolution. However, despite this tremendous potential, challenges persist due to the high complexity in both the ADC molecules and the related biological systems. This review summarizes the up-to-date LC-MS-based strategies in ADC analysis and discusses the challenges and opportunities in this rapidly-evolving field.
在过去几年中，抗体药物偶联物（ADC）的发展取得了巨大进展。因此，在生物系统中对ADC进行全面分析对于支持这些药物的发现、开发和评估至关重要。液相色谱-质谱联用（LC-MS）由于其多重分析能力、快速的方法开发以及能够以高分子分辨率分析从小分子载荷到完整蛋白质等各种目标，已成为一种在广泛场景下进行ADC分析的有前途且通用的工具。然而，尽管有这种巨大潜力，但由于ADC分子和相关生物系统的高度复杂性，挑战依然存在。本综述总结了基于LC-MS的ADC分析最新策略，并讨论了这个快速发展领域中的挑战与机遇。
