Garcon N, Gregoriadis G, Taylor M, Summerfield J
Medical Research Council Group, Royal Free Hospital School of Medicine, London, U.K.
Immunology. 1988 Aug;64(4):743-5.
The effect of the ligand mannosylated albumin, covalently coupled to the surface of tetanus toxoid-containing dehydration-rehydration vesicles (DRV), on their adjuvanticity was investigated. Toxoid-containing DRV coated with mannosylated albumin bound in vitro to mouse (BALB/c) peritoneal macrophages selectively and to a greater extent than non-mannosylated DRV. ELISA assays of anti-toxoid IgG1 and IgG2b in the sera of BALB/c mice immunized with various toxoid-containing DRV preparations suggest that mannosylated liposomes are superior in immunoadjuvant action to conventional ones. Such targeted adjuvanticity appears to be influenced by the number of ligand molecules available on the surface of liposomes rather than the number of mannose residues on albumin.
研究了共价偶联到含破伤风类毒素的脱水再水化囊泡（DRV）表面的配体甘露糖化白蛋白对其佐剂活性的影响。用甘露糖化白蛋白包被的含类毒素DRV在体外与小鼠（BALB/c）腹腔巨噬细胞选择性结合，且比未甘露糖化的DRV结合程度更高。对用各种含类毒素DRV制剂免疫的BALB/c小鼠血清中的抗类毒素IgG1和IgG2b进行ELISA分析表明，甘露糖化脂质体在免疫佐剂作用方面优于传统脂质体。这种靶向佐剂活性似乎受脂质体表面可用配体分子数量的影响，而非白蛋白上甘露糖残基的数量。
