Hambleton P, Miller P
Roussel Laboratories Ltd., Swindon, Wilts., UK.
Int Arch Allergy Appl Immunol. 1988;87(1):70-5. doi: 10.1159/000234651.
The development of a novel immune-based model of inflammation in the subcutaneous air pouch of the rat is described. Rats were sensitized by injection with methylated bovine serum albumin emulsified with Freund's complete adjuvant and challenged 14 days later by injection of heat-aggregated antigen in saline into a 6-day subcutaneous air pouch. Time course studies revealed a peak polymorph cell infiltration at 24 h with no exudate formation, followed by a separate mononuclear cell response, rapid exudate formation, and granuloma formation over the 2- to 7-day period. Separate peaks of increased vascular permeability were observed in the acute and chronic phases of the response. This reaction was considered to be an example of delayed-type hypersensitivity based on its extended time course, the characteristic time course of oedema formation in foot-pad-challenged rats, and the failure of serum from sensitized rats to elicit a reversed passive Arthus-type reaction. The sensitization schedule was also shown to enhance the acute inflammatory response to carrageenan. In addition, the 'flare-up' reaction to secondary antigenic challenge was investigated in 7-day postchallenge lesions.
本文描述了一种在大鼠皮下气囊中建立的基于免疫的新型炎症模型。通过注射用弗氏完全佐剂乳化的甲基化牛血清白蛋白使大鼠致敏，并在14天后通过将热聚集抗原注射到6天龄的皮下气囊中进行激发。时间进程研究显示，在24小时出现多形核细胞浸润高峰，无渗出物形成，随后在2至7天期间出现单独的单核细胞反应、快速渗出物形成和肉芽肿形成。在反应的急性期和慢性期观察到血管通透性增加的单独高峰。基于其延长的时间进程、足垫激发大鼠水肿形成的特征性时间进程以及致敏大鼠血清未能引发反向被动阿瑟斯型反应，该反应被认为是迟发型超敏反应的一个例子。致敏方案还显示可增强对角叉菜胶的急性炎症反应。此外，还在激发后7天的损伤中研究了对二次抗原激发的“爆发”反应。
