Isaji M, Naito J
Second Research Laboratories, Kissei Pharmaceutical Co. Ltd, Japan.
Int J Exp Pathol. 1992 Apr;73(2):231-9.
Inflammatory reactions were compared in the air pouch and the CMC pouch. Inflammation was induced by injection of CMC as a non-specific irritant, lipopolysaccharide (LPS) as an activator of macrophages, and methylated bovine serum albumin (m-BSA) as an inducer of delayed type hypersensitivity. There was no prominent difference in the inflammatory reactions following injection of 2% CMC solution into a 4-day-old air pouch and a 3-day-old CMC pouch. On the other hand, injection of 4 ml of 100 ng/ml LPS into each of the pouches enhanced the inflammatory reactions in the CMC pouch several-fold compared with those in the air pouch. A similar tendency was found in the case of an injection of 1 ml of 1 mg/ml m-BSA in rats sensitized with 1 mg of m-BSA. The enhanced inflammatory reactions induced by the injection of LPS or m-BSA were inhibited by dexamethasone, but not by indomethacin. These results indicate that the enhanced inflammatory reactions induced by CMC are related to lining tissue formation, which was a common characteristic in both pouches. Enhanced inflammatory reactions following injection of LPS and m-BSA were related to the activation of macrophages and newly formed blood vessels, which were not characteristic features in the air pouch, but were in the CMC pouch, in addition to the lining tissue. Cyclo-oxygenase products were not associated with the reactions.
对气袋和羧甲基纤维素（CMC）袋中的炎症反应进行了比较。通过注射作为非特异性刺激物的CMC、作为巨噬细胞激活剂的脂多糖（LPS）以及作为迟发型超敏反应诱导剂的甲基化牛血清白蛋白（m-BSA）来诱导炎症。将2% CMC溶液注射到4日龄气袋和3日龄CMC袋后，炎症反应没有显著差异。另一方面，向每个袋中注射4 ml 100 ng/ml LPS后，与气袋相比，CMC袋中的炎症反应增强了几倍。在用1 mg m-BSA致敏的大鼠中注射1 ml 1 mg/ml m-BSA时也发现了类似的趋势。注射LPS或m-BSA诱导的增强炎症反应被地塞米松抑制，但未被吲哚美辛抑制。这些结果表明，CMC诱导的增强炎症反应与衬里组织形成有关，这是两个袋的共同特征。注射LPS和m-BSA后增强的炎症反应与巨噬细胞的激活和新形成的血管有关，这些不是气袋的特征，但除了衬里组织外，是CMC袋的特征。环氧化酶产物与这些反应无关。
