Yoshino S, Bacon P A, Blake D R, Scott D L, Wainwright A C, Walton K W
Br J Exp Pathol. 1984 Apr;65(2):201-14.
Continuing antigen-induced inflammation was established in a subcutaneous air pouch in rats by recurrent local challenge. The animals were sensitized using bovine serum albumin in Freund's complete adjuvant and were challenged 14 days later by injection of the antigen in a solution containing sodium carboxymethylcellulose into the air pouch to produce allergic inflammation. A single antigenic challenge induced acute inflammation with a predominantly polymorph infiltration in the first 48 h. Later samples showed a low-grade mononuclear response which persisted for 4-5 days. Repeated challenge produced chronic inflammation with an accentuated mononuclear response. Connective tissue activation involving fibronectin and collagen was seen as the inflammation progressed, and this was associated with production of ferritin by mononuclear cells. Discontinuation of challenge injections resulted in resolution of the granuloma. We suggest this model can be used to investigate the mechanisms involved in chronic inflammatory diseases with an immunological component and to evaluate the effects of therapeutic intervention upon chronic allergic inflammation.
通过反复局部激发在大鼠皮下气囊中建立持续抗原诱导的炎症。动物用弗氏完全佐剂中的牛血清白蛋白致敏，14天后通过将抗原注射到含有羧甲基纤维素钠的溶液中的气囊中进行激发，以产生过敏性炎症。单次抗原激发在最初48小时内诱导急性炎症，主要为多形核细胞浸润。后来的样本显示低度单核细胞反应持续4 - 5天。反复激发产生慢性炎症，单核细胞反应加剧。随着炎症进展，可见涉及纤连蛋白和胶原蛋白的结缔组织活化，这与单核细胞产生铁蛋白有关。停止激发注射导致肉芽肿消退。我们认为该模型可用于研究具有免疫成分的慢性炎症性疾病所涉及的机制，并评估治疗干预对慢性过敏性炎症的影响。
