CD19CD24CD27调节性B细胞的比例可预测肝移植中急性移植物排斥反应的发生。
The proportion of CD19CD24CD27 regulatory B cells predicts the occurrence of acute allograft rejection in liver transplantation.
作者信息
Zhou Haoming, Zhan Feng, Zhang Hui, Gu Jian, Mu Xiaoxin, Gao Ji, Rao Jianhua, Ji Guwei, Ni Xuhao, Lu Ling, Xia Yongxiang
机构信息
Translational Medicine Research Center of Affiliated Jiangning Hospital, Liver Transplantation Center of First Affiliated Hospital, and Collaborative Innovation Center for Cancer Medicine, Nanjing Medical University, Nanjing 210029, China.
Key Laboratory of Liver Transplantation, Chinese Academy of Medical Sciences, Nanjing 210029, China.
出版信息
Ann Transl Med. 2019 Sep;7(18):465. doi: 10.21037/atm.2019.08.05.
BACKGROUND
Regulatory B cells (Bregs) play an essential role in inflammation and transplant tolerance. Several studies have reported a decreased number of Bregs in renal transplant patients with graft rejection. However, little is known about their role in the liver alloresponse.
METHODS
To investigate whether the circulating Bregs have been associated with acute allograft rejection (AR) in liver transplantation patients, 19 patients receiving liver allografts from donation after cardiac death (DCD) donors were retrospectively studied.
RESULTS
The postoperative proportions of circulating CD19CD24CD38 transitional Bregs (tBregs) and CD19CD24CD27 memory Bregs (mBregs) in patients diagnosed with AR (AR group) and other patients with stable allograft liver function (SF group) were evaluated using flow cytometry (FCM) analysis. Results showed that while no significant changes were found regarding both the tBreg and mBreg, proportions across all time points in the SF group, the AR group showed significantly decreased proportions of mBregs. All of the five AR patients responded fine to the treatments, and the proportions of mBregs increased significantly after anti-rejection therapies. In addition, AR was suspected in four recipients, but gradually they were diagnosed with hemolytic or obstructive jaundice and showed no decrease in the proportion of mBregs.
CONCLUSIONS
For the first time, our results suggested the potential role of a decreased proportion of circulating mBregs in predicting AR in patients with post liver transplantation.
背景
调节性B细胞（Bregs）在炎症和移植耐受中起重要作用。多项研究报道，发生移植物排斥反应的肾移植患者体内Bregs数量减少。然而，它们在肝脏同种异体反应中的作用尚不清楚。
方法
为了研究循环Bregs是否与肝移植患者的急性移植物排斥反应（AR）相关，我们对19例接受心脏死亡后供体（DCD）肝脏移植的患者进行了回顾性研究。
结果
采用流式细胞术（FCM）分析评估了诊断为AR的患者（AR组）和其他移植肝功能稳定的患者（SF组）术后循环CD19⁺CD24⁺CD38⁺过渡性Bregs（tBregs）和CD19⁺CD24⁺CD27⁺记忆性Bregs（mBregs）的比例。结果显示，虽然SF组在所有时间点tBregs和mBregs的比例均无显著变化，但AR组mBregs的比例显著降低。所有5例AR患者对治疗反应良好，抗排斥治疗后mBregs的比例显著增加。此外，4例受者疑似发生AR，但随后逐渐被诊断为溶血性或梗阻性黄疸，mBregs的比例未降低。
结论
我们的结果首次表明，循环mBregs比例降低在预测肝移植术后患者AR方面具有潜在作用。
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