长链非编码 RNA LINC00974 通过下调 miR-122 来上调口腔鳞状细胞癌中的 RhoA。
LncRNA LINC00974 Downregulates miR-122 to Upregulate RhoA in Oral Squamous Cell Carcinoma.
机构信息
Department of Stomatology, The First Affiliated Hospital of Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou, China.
Department of Stomatology, Baotou Eighth Hospital, Baotou, China.
出版信息
Cancer Biother Radiopharm. 2021 Feb;36(1):18-22. doi: 10.1089/cbr.2019.2907. Epub 2019 Nov 8.
LncRNA LINC00974 participates in oral fibrogenesis, indicating possible involvement in other oral diseases. The authors found that LINC00974 was upregulated in oral squamous cell carcinoma (OSCC) and predicted poor survival. In OSCC tissues, LINC00974 was inversely correlated with miR-122 and positively correlated with RhoA. In OSCC cells, LINC00974 overexpression resulted in upregulated, whereas overexpression of miR-122 led to downregulated RhoA. Moreover, downregulated miR-122 was observed after LINC00974 overexpression, whereas LINC00974 expression was not significantly affected by miR-122 overexpression. In invasion and migration assay, miR-122 overexpression resulted in reduced, whereas LINC00974 and RhoA overexpression resulted in increased rate of cancer cell migration and invasion. In addition, miR-122 overexpression reduced the effects of LINC00974 overexpression. Therefore, LINC00974 can downregulate miR-122 to upregulate RhoA in OSCC, thereby promoting cell invasion and migration.
LncRNA LINC00974 参与口腔纤维化，表明其可能参与其他口腔疾病。作者发现 LINC00974 在口腔鳞状细胞癌（OSCC）中上调，并预测预后不良。在 OSCC 组织中，LINC00974 与 miR-122 呈负相关，与 RhoA 呈正相关。在 OSCC 细胞中，过表达 LINC00974 导致 RhoA 上调，而过表达 miR-122 则导致 RhoA 下调。此外，过表达 LINC00974 后观察到 miR-122 下调，而 miR-122 过表达对 LINC00974 表达没有显著影响。在侵袭和迁移实验中，过表达 miR-122 导致癌细胞迁移和侵袭率降低，而过表达 LINC00974 和 RhoA 则导致癌细胞迁移和侵袭率升高。此外，过表达 miR-122 降低了 LINC00974 过表达的作用。因此，LINC00974 可以下调 miR-122 以上调 OSCC 中的 RhoA，从而促进细胞侵袭和迁移。
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