Feng Zhi, Li Xu, Qiu Minglian, Luo Ronggang, Lin Jianbo, Liu Bo
Thoracic Surgery Department, First Affiliated Hospital, Fujian Medical University, Fuzhou City, People's Republic of China.
Cancer Biother Radiopharm. 2020 Feb;35(1):66-71. doi: 10.1089/cbr.2019.2926. Epub 2019 Nov 8.
EGFR-AS1 has been characterized as an oncogenic lncRNA in many types of cancers, while its roles in esophageal squamous cell carcinoma (ESCC) are unknown. Their data showed that EGFR-AS1 and ROCK1 were upregulated in ESCC and positively correlated. Survival analysis showed that high EGFR-AS1 and ROCK1 expression levels predicted poor survival. In ESCC cells, EGFR-AS1 overexpression led to upregulated ROCK1, while miR-145 overexpression led to downregulated ROCK1 and reduced effects of EGFR-AS1 overexpression. Bioinformatics analysis showed that miR-145 may bind EGFR-AS1, while overexpression of EGFR-AS1 and miR-145 did not significantly affect each other. In esophageal squamous cell carcinoma (ESCC) cells, EGFR-AS1 and ROCK1 overexpression mediated the increased rates of ECSS cell invasion and migration. Overexpression of miR-145 played an opposite role and attenuated the effects of EGFR-AS1 overexpression. Therefore, EGFR-AS1 may upregulate ROCK1 by sponging miR-145 to promote ESCC cell invasion and migration.
表皮生长因子受体反义RNA1（EGFR-AS1）在多种癌症中已被鉴定为一种致癌长链非编码RNA（lncRNA），但其在食管鳞状细胞癌（ESCC）中的作用尚不清楚。他们的数据显示，EGFR-AS1和Rho相关卷曲螺旋蛋白激酶1（ROCK1）在ESCC中上调且呈正相关。生存分析表明，EGFR-AS1和ROCK1的高表达水平预示着较差的生存率。在ESCC细胞中，EGFR-AS1过表达导致ROCK1上调，而miR-145过表达导致ROCK1下调并减弱了EGFR-AS1过表达的作用。生物信息学分析表明，miR-145可能与EGFR-AS1结合，而EGFR-AS1和miR-145的过表达彼此之间没有显著影响。在食管鳞状细胞癌细胞中，EGFR-AS1和ROCK1过表达介导了ESCC细胞侵袭和迁移率的增加。miR-145过表达则起相反作用并减弱了EGFR-AS1过表达的影响。因此，EGFR-AS1可能通过吸附miR-145上调ROCK1，从而促进ESCC细胞的侵袭和迁移。
