The Saban Research Institute of Children's Hospital Los Angeles, Los Angeles, United States.
Department of Biology, California State University of San Bernardino, San Bernardino, United States.
Elife. 2019 Nov 8;8:e42762. doi: 10.7554/eLife.42762.
The cardiac lymphatic vascular system and its potentially critical functions in heart patients have been largely underappreciated, in part due to a lack of experimentally accessible systems. We here demonstrate that cardiac lymphatic vessels develop in young adult zebrafish, using coronary arteries to guide their expansion down the ventricle. Mechanistically, we show that in mutants with defective coronary artery development, cardiac lymphatic vessels fail to expand onto the ventricle. In regenerating adult zebrafish hearts the lymphatic vasculature undergoes extensive lymphangiogenesis in response to a cryoinjury. A significant defect in reducing the scar size after cryoinjury is observed in zebrafish with impaired Vegfc/Vegfr3 signaling that fail to develop intact cardiac lymphatic vessels. These results suggest that the cardiac lymphatic system can influence the regenerative potential of the myocardium.
心脏的淋巴血管系统及其在心脏病患者中的潜在关键功能在很大程度上被低估了，部分原因是缺乏实验上可及的系统。我们在这里证明，心脏淋巴管在年轻的成年斑马鱼中发育，利用冠状动脉引导其向下扩展到心室。从机制上讲，我们表明在冠状动脉发育不良的 突变体中，心脏淋巴管未能扩展到心室上。在再生的成年斑马鱼心脏中，淋巴血管系统在受到冷冻损伤后会发生广泛的淋巴管生成。在 Vegfc/Vegfr3 信号传导受损的斑马鱼中，观察到冷冻损伤后疤痕大小减少的显著缺陷，这些斑马鱼无法形成完整的心脏淋巴管。这些结果表明，心脏淋巴系统可以影响心肌的再生潜力。
