Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, International Campus, Tehran, Iran.
Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
J Biomed Mater Res A. 2020 Mar;108(3):545-556. doi: 10.1002/jbm.a.36835. Epub 2019 Nov 20.
Wound healing is known as one of the most complicated biological processes for injured skin caused by surgical, trauma, burns, or diabetic diseases, which causes a nonfunctioning mass of fibrotic tissue. Recent reports have suggested that exosomes (EXOs) secreted by this type of stem cells may contribute to their paracrine effect. In this study, the EXOs were isolated from the supernatant of cultured adipose-derived stem cells (ADSCs) via ultracentrifugation and filtration. The EXO loaded in the alginate-based hydrogel was used as a bioactive scaffold to preserve the EXO in the wound site in the animal model. The physical and biochemical properties of EXO loaded Alg hydrogel were characterized and results proved that fabricated structure was biodegradable and biocompatible. This bioactive wound dressing technique has significantly improved wound closure, collagen synthesis, and vessel formation in the wound area. Results offer a new viewpoint and a cell-free therapeutic strategy, for wound healing through the application of the composite structure of EXO encapsulated in alginate hydrogel.
伤口愈合是由手术、创伤、烧伤或糖尿病等原因导致的受伤皮肤的一种最复杂的生物学过程，它会导致无功能的纤维组织块。最近的报告表明，这种干细胞分泌的外泌体（EXOs）可能有助于它们的旁分泌作用。在这项研究中，通过超速离心和过滤从培养的脂肪来源干细胞（ADSCs）的上清液中分离 EXO。负载在藻酸盐水凝胶中的 EXO 被用作生物活性支架，以将 EXO 保留在动物模型中的伤口部位。对负载 EXO 的藻酸盐水凝胶的物理和生化特性进行了表征，结果证明所构建的结构是可生物降解和生物相容的。这种活性创面敷料技术显著提高了创面的闭合、胶原合成和血管形成。结果为通过应用负载在藻酸盐水凝胶中的 EXO 的复合结构进行创面愈合提供了一个新的观点和无细胞治疗策略。
