Department of Pharmacology, School of Pharmacy, China Medical University, Shenyang, PR China.
Drug Deliv. 2021 Dec;28(1):884-893. doi: 10.1080/10717544.2021.1912210.
Full thickness cutaneous wound therapy and regeneration remains a critical challenge in clinical therapeutics. Recent reports have suggested that mesenchymal stem cells exosomes therapy is a promising technology with great potential to efficiently promote tissue regeneration. Multifunctional hydrogel composed of both synthetic materials and natural materials is an effective carrier for exosomes loading. Herein, we constructed a biodegradable, dual-sensitive hydrogel encapsulated human umbilical cord-mesenchymal stem cells (hUCMSCs) derived exosomes to facilitate wound healing and skin regeneration process. The materials characterization, exosomes identification, and full-thickness cutaneous wound healing effect of the hydrogels were performed and evaluated. The results demonstrated the exosomes loaded hydrogel had significantly improved wound closure, re-epithelialization rates, collagen deposition in the wound sites. More skin appendages were observed in exosomes loaded hydrogel treated wound, indicating the potential to achieve complete skin regeneration. This study provides a new access for complete cutaneous wound regeneration via a genipin crosslinked dual-sensitive hydrogel loading hUCMSCs derived exosomes.
全层皮肤创面治疗和再生仍然是临床治疗的一个关键挑战。最近的报告表明，间充质干细胞外泌体治疗是一种很有前途的技术，具有高效促进组织再生的巨大潜力。由合成材料和天然材料组成的多功能水凝胶是外泌体负载的有效载体。本文构建了一种可生物降解的、双重敏感的水凝胶，封装了人脐带间充质干细胞（hUCMSCs）衍生的外泌体，以促进创面愈合和皮肤再生过程。对水凝胶的材料特性、外泌体鉴定以及全层皮肤创面愈合效果进行了评估。结果表明，负载外泌体的水凝胶显著提高了创面闭合率、再上皮化率和胶原在创面的沉积。在外泌体负载水凝胶处理的创面中观察到更多的皮肤附属物，表明有可能实现完全的皮肤再生。本研究通过基因交联的双重敏感水凝胶负载 hUCMSCs 衍生的外泌体为全层皮肤创面再生提供了一种新方法。
