一项关于患有冠状动脉粥样硬化男性的动脉粥样硬化斑块的蛋白质组学研究。
A Proteomic Study of Atherosclerotic Plaques in Men with Coronary Atherosclerosis.
作者信息
Stakhneva Ekaterina M, Meshcheryakova Irina A, Demidov Evgeny A, Starostin Konstantin V, Sadovski Evgeny V, Peltek Sergey E, Voevoda Michael I, Chernyavskii Alexander M, Volkov Alexander M, Ragino Yuliya I
机构信息
Research Institute of Internal and Preventive Medicine - Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 630089 Novosibirsk, Russia.
Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, Russia.
出版信息
Diagnostics (Basel). 2019 Nov 7;9(4):177. doi: 10.3390/diagnostics9040177.
BACKGROUND
To study the changes in protein composition of atherosclerotic plaques at different stages of their development in coronary atherosclerosis using proteomics.
METHODS
The object of research consisted of homogenates of atherosclerotic plaques from coronary arteries at different stages of development, obtained from 15 patients. Plaque proteins were separated by two-dimensional electrophoresis. The resultant protein spots were identified by the matrix-assisted laser desorption ionization method with peptide mass mapping.
RESULTS
Groups of differentially expressed proteins, in which the amounts of proteins differed more than twofold ( < 0.05), were identified in pools of homogenates of atherosclerotic plaques at three stages of development. The amounts of the following proteins were increased in stable atherosclerotic plaques at the stage of lipidosis and fibrosis: vimentin, tropomyosin β-chain, actin, keratin, tubulin β-chain, microfibril-associated glycoprotein 4, serum amyloid P-component, and annexin 5. In plaques at the stage of fibrosis and calcification, the amounts of mimecan and fibrinogen were increased. In unstable atherosclerotic plaque of the necrotic-dystrophic type, the amounts of human serum albumin, mimecan, fibrinogen, serum amyloid P-component and annexin were increased.
CONCLUSION
This proteomic study identifies the proteins present in atherosclerotic plaques of coronary arteries by comparing their proteomes at three different stages of plaque development during coronary atherosclerosis.
背景
运用蛋白质组学研究冠状动脉粥样硬化斑块在不同发展阶段蛋白质组成的变化。
方法
研究对象为从15例患者获取的处于不同发展阶段的冠状动脉粥样硬化斑块匀浆。通过二维电泳分离斑块蛋白质。采用基质辅助激光解吸电离法结合肽质量图谱鉴定所得蛋白质斑点。
结果
在粥样硬化斑块发展的三个阶段的匀浆样本中，鉴定出了差异表达蛋白组，其中蛋白质含量差异超过两倍（<0.05）。在脂变和纤维化阶段的稳定粥样硬化斑块中，波形蛋白、原肌球蛋白β链、肌动蛋白、角蛋白、微管蛋白β链、微原纤维相关糖蛋白4、血清淀粉样蛋白P成分和膜联蛋白5的含量增加。在纤维化和钙化阶段的斑块中， mimecan和纤维蛋白原的含量增加。在坏死营养不良型不稳定粥样硬化斑块中，人血清白蛋白、mimecan、纤维蛋白原、血清淀粉样蛋白P成分和膜联蛋白的含量增加。
结论
这项蛋白质组学研究通过比较冠状动脉粥样硬化斑块发展的三个不同阶段的蛋白质组来鉴定冠状动脉粥样硬化斑块中存在的蛋白质。
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