College of Forestry, Henan University of Science and Technology, Luoyang, 471023, Henan Province, China.
Henan Engineering Technology Research Center of Quality Regulation and Controlling of Horticultural Plants, Luoyang, 471023, Henan Province, China.
BMC Genomics. 2019 Nov 8;20(1):825. doi: 10.1186/s12864-019-6204-1.
5-Azacytidine (5-azaC) promotes the development of 'Kyoho' grape berry but the associated changes in gene expression have not been reported. In this study, we performed transcriptome analysis of grape berry at five developmental stages after 5-azaC treatment to elucidate the gene expression networks controlling berry ripening.
The expression patterns of most genes across the time series were similar between the 5-azaC treatment and control groups. The number of differentially expressed genes (DEGs) at a given developmental stage ranged from 9 (A3_C3) to 690 (A5_C5). The results indicated that 5-azaC treatment had not very great influences on the expressions of most genes. Functional annotation of the DEGs revealed that they were mainly related to fruit softening, photosynthesis, protein phosphorylation, and heat stress. Eight modules showed high correlation with specific developmental stages and hub genes such as PEROXIDASE 4, CAFFEIC ACID 3-O-METHYLTRANSFERASE 1, and HISTONE-LYSINE N-METHYLTRANSFERASE EZA1 were identified by weighted gene correlation network analysis.
5-AzaC treatment alters the transcriptional profile of grape berry at different stages of development, which may involve changes in DNA methylation.
5-氮杂胞苷（5-azaC）能促进“巨峰”葡萄果实的发育，但相关基因表达变化尚未见报道。本研究通过对 5-azaC 处理后五个发育阶段的葡萄果实进行转录组分析，阐明了控制果实成熟的基因表达网络。
在整个时间序列中，大多数基因在 5-azaC 处理组和对照组之间的表达模式相似。在特定发育阶段差异表达基因（DEGs）的数量范围为 9（A3_C3）到 690（A5_C5）。结果表明，5-azaC 处理对大多数基因的表达没有很大影响。对 DEGs 的功能注释表明，它们主要与果实软化、光合作用、蛋白质磷酸化和热应激有关。八个模块与特定发育阶段高度相关，通过加权基因共相关网络分析鉴定出了与模块相关的关键基因，如过氧化物酶 4、咖啡酸 3-O-甲基转移酶 1 和组蛋白赖氨酸 N-甲基转移酶 EZA1。
5-azaC 处理改变了不同发育阶段葡萄果实的转录谱，这可能涉及 DNA 甲基化的变化。
