College of Forestry, Henan University of Science and Technology, Luoyang 471023, Henan Province, China.
Henan Engineering Technology Research Center of Quality Regulation and Controlling of Horticultural Plants, Luoyang 471023, Henan Province, China.
Genes (Basel). 2019 Jul 6;10(7):514. doi: 10.3390/genes10070514.
Previous study has demonstrated that the riboflavin treatment promoted the early ripening of the 'Kyoho' grape berry. However, the molecular mechanism causing this was unclear. In order to reveal the regulation mechanism of riboflavin treatment on grape berry development and ripening, the different berry developmental stages of the 'Kyoho' berry treated with 0.5 mmol/L of riboflavin was sampled for transcriptome profiling. RNA-seq revealed that 1526 and 430 genes were up-regulated and down-regulated, respectively, for the comparisons of the treatment to the control. TCseq analysis showed that the expression patterns of most of the genes were similar between the treatment and the control, except for some genes that were related to the chlorophyll metabolism, photosynthesis-antenna proteins, and photosynthesis, which were revealed by the enrichment analysis of Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). The differentially expressed genes and weighted gene co-expression network analysis (WGCNA) analysis identified some significantly differentially expressed genes and some hub genes, including up-regulation of the photosynthesis-related and growth and development-related ; and down-regulation of the oxidative stress-related and berry softening-related and . The results suggested that the riboflavin treatment resulted in the variations of the expression levels of these genes, and then led to the early ripening of the 'Kyoho' berry.
先前的研究表明，核黄素处理可促进“巨峰”葡萄果实的早期成熟。然而，导致这种情况的分子机制尚不清楚。为了揭示核黄素处理对葡萄果实发育和成熟的调控机制，对用 0.5mmol/L 核黄素处理的“巨峰”葡萄不同浆果发育阶段进行了转录组谱分析。RNA-seq 结果表明，处理组与对照组相比，分别有 1526 个基因上调和 430 个基因下调。TCseq 分析表明，大多数基因的表达模式在处理组和对照组之间相似，除了一些与叶绿素代谢、光合作用天线蛋白和光合作用相关的基因，这些基因通过基因本体论（GO）和京都基因与基因组百科全书（KEGG）的富集分析显示。差异表达基因和加权基因共表达网络分析（WGCNA）分析鉴定了一些显著差异表达的基因和一些枢纽基因，包括与光合作用相关的 和生长发育相关的 上调；以及与氧化应激相关的 和浆果软化相关的 和 下调。结果表明，核黄素处理导致这些基因表达水平的变化，进而导致“巨峰”葡萄的早期成熟。
