Francis G E, Kennedy P G, Hoffbrand B I, Berney J J, Hoffbrand A V
Clin Lab Haematol. 1979;1(3):203-14. doi: 10.1111/j.1365-2257.1979.tb00469.x.
Soft agar culture of bone marrow cells was used to investigate abnormalities of granulopoiesis in a patient with acquired anaemia and neutropenia which responded to immunosuppressive therapy. Results showed that the patient's peripheral blood leucocytes inhibited granulopoiesis in vitro. Variation in the response of different target marrows suggested that inhibition was not due to direct action on granulocyte-monocyte committed stem cells but was directed against or mediated through the cells in target marrow samples which produce granulopoietic stimulators. Cell separation showed that the inhibitory activity was associated with the patient's mononuclear cells rather than neutrophils. A tentative explanation of the mechanism of production of the anaemia is also offered.
采用骨髓细胞软琼脂培养法，对一名获得性贫血和中性粒细胞减少且对免疫抑制治疗有效的患者的粒细胞生成异常情况进行了研究。结果显示，该患者的外周血白细胞在体外抑制粒细胞生成。不同靶骨髓反应的差异表明，抑制并非由于对粒-单核系定向干细胞的直接作用，而是针对或通过靶骨髓样本中产生粒细胞生成刺激因子的细胞介导的。细胞分离显示，抑制活性与患者的单核细胞而非中性粒细胞相关。本文还对贫血产生机制提出了一个初步解释。
