Dow Lachlan, Morrissey Kathryn Lee, Willems Anne, Kroth Peter G
Plant Ecophysiology, Department of Biology, University of Konstanz, 10 Universitaetstrasse, 78467 Konstanz, Germany.
Department of Biology, Phycology Research Group, Ghent University, Campus Sterre, Krijgslaan 281/S8, 9000 Ghent, Belgium Ghent, Belgium.
Mar Genomics. 2020 Aug;52:100720. doi: 10.1016/j.margen.2019.100720. Epub 2019 Nov 5.
Bacteria have been shown to be involved in different species-specific interactions with eukaryotic algae such as diatoms, impacting important ecosystem processes. Recently, a strain assigned to Dyadobacter, named 'species 32', has been shown to be involved in a number of ecologically relevant diatom processes, such as biofilm formation or growth enhancement, depending on the diatom species. This bacterium was originally isolated from a culture of freshwater benthic diatoms that originated from an epilithic biofilm, in which both bacteria and diatoms coexist. A single complete circular chromosome of Dyadobacter sp. 32 was assembled with a length of 7,101,228 bp, containing 6062 protein coding genes and 3 rRNA operons. A number of interesting genetic features were found, such as a putative zeaxanthin biosynthetic gene cluster. A large number of polysaccharide utilizing gene clusters were also detected, along with genes potentially acquired from other bacteria through horizontal gene transfer, and genes previously identified in other algae-bacteria interactions. These data serve to increase our understanding of specific interactions within freshwater biofilms, and identify a number of gene targets with which to study the molecular basis of diatom-bacteria interactions.
细菌已被证明参与与真核藻类（如硅藻）的不同物种特异性相互作用，影响重要的生态系统过程。最近，一种被归类为双杆菌属的菌株，名为“物种32”，已被证明参与了许多与硅藻生态相关的过程，如生物膜形成或生长促进，这取决于硅藻的种类。这种细菌最初是从一种源自附石生物膜的淡水底栖硅藻培养物中分离出来的，在该生物膜中细菌和硅藻共存。双杆菌属物种32的一条完整环状染色体被组装出来，长度为7,101,228 bp，包含6062个蛋白质编码基因和3个rRNA操纵子。发现了许多有趣的遗传特征，如一个假定的玉米黄质生物合成基因簇。还检测到大量利用多糖的基因簇，以及可能通过水平基因转移从其他细菌获得的基因，以及先前在其他藻菌相互作用中鉴定出的基因。这些数据有助于增进我们对淡水生物膜内特定相互作用的理解，并确定一些用于研究硅藻-细菌相互作用分子基础的基因靶点。
