Dahal Ram Hari, Kim Jaisoo
Department of Life Science, College of Natural Sciences, Kyonggi University, Suwon, Gyeonggi-Do, 16227, South Korea.
Arch Microbiol. 2018 Sep;200(7):1067-1074. doi: 10.1007/s00203-018-1521-3. Epub 2018 May 8.
Two strains of bacteria designated strains S-53 and A27 were isolated from forest soil and subjected to polyphasic characterization. Cells were aerobic, Gram-staining-negative, catalase- and oxidase- positive, non-motile, non-spore-forming, rod-shaped, and yellow-pigmented. Flexirubin-type pigments were present. Both strains were positive for PNPG, hydrolysed casein, and tyrosine. A phylogenetic analysis based on its 16S rRNA gene sequence revealed that strains S-53 and A27 formed a lineage within the family Cytophagaceae that were distinct from various members of the genus Dyadobacter (98.9-93.2% sequence similarity). Closest member for strain S-53 was Dyadobacter jejuensis AM1R11 (95.7%) and for A27 Dyadobacter endophyticus 65 (98.9%). The predominant respiratory quinone was MK-7 for both strains. The major polar lipid for both strains was phosphatidylethanolamine. The major cellular fatty acids for both strains were summed feature 3 (Cω7c and/or Cω6c), iso-C, Cω5c, and C. The DNA G+C content of strains ranges from 46.5 to 48.7 mol%. On the basis of phenotypic, genotypic, chemotaxonomic, and phylogenetic analysis, both strains S-53 and A27 represent a novel member in the genus Dyadobacter, for which the name Dyadobacter flavus sp. nov. and Dyadobacter terricola sp. nov. are proposed, respectively. The type strain of D. flavus is S-53 (= KEMB 9005-541 = KACC 19149 = NBRC 112681) and type strain of D. terricola is A27 (= KEMB 9005-524 = KACC 19147 = NBRC 112680).
从森林土壤中分离出两株细菌，分别命名为S-53菌株和A27菌株，并对其进行了多相表征。细胞为需氧型，革兰氏染色阴性，过氧化氢酶和氧化酶阳性，无运动性，不形成芽孢，杆状，且带有黄色色素。存在Flexirubin型色素。两株菌对PNPG、水解酪蛋白和酪氨酸均呈阳性反应。基于其16S rRNA基因序列的系统发育分析表明，S-53菌株和A27菌株在噬纤维菌科内形成了一个分支，与Dyadobacter属的各个成员不同（序列相似性为98.9 - 93.2%）。S-53菌株最接近的成员是济州岛Dyadobacter jejuensis AM1R11（95.7%），A27菌株最接近的成员是内生Dyadobacter endophyticus 65（98.9%）。两株菌的主要呼吸醌均为MK-7。两株菌的主要极性脂均为磷脂酰乙醇胺。两株菌的主要细胞脂肪酸均为总和特征3（Cω7c和/或Cω6c）、异-C、Cω5c和C。菌株的DNA G+C含量范围为46.5至48.7 mol%。基于表型、基因型、化学分类和系统发育分析，S-53菌株和A27菌株均代表Dyadobacter属的一个新成员，分别提议将其命名为黄Dyadobacter flavus sp. nov.和土生Dyadobacter terricola sp. nov.。黄Dyadobacter flavus的模式菌株为S-53（= KEMB 9005-541 = KACC 19149 = NBRC 112681），土生Dyadobacter terricola的模式菌株为A27（= KEMB 9005-524 = KACC 19147 = NBRC 112680）。
