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新种和新种,来自饮用水。

sp. nov. and sp. nov., from drinking water.

机构信息

Departamento de Microbiología y Ecología, Universitat de València, Spain.

出版信息

Int J Syst Evol Microbiol. 2024 Nov;74(11). doi: 10.1099/ijsem.0.006570.

Abstract

The purpose of this study is to better characterize and complete the classification of two bacterial strains, CECT 9275 and CECT 9623, isolated from drinking water systems and affiliated to the genus by partial 16S rRNA gene sequence comparison. Hence, we report here the phenotypic, genomic and phylogenetic characterization performed on these strains. Both strains grow on R2A agar forming mucous, bright yellow colonies, developing at 26 °C in 48 h. They produce flexirubin and are oxidase and catalase positive, mesophilic and non-halophilic. The cells of strain CECT 9275 are curved rods mainly associated in pairs, forming nearly closed rings or resembling the shape of the number three, to long spirals resembling a corkscrew. Its draft genome has an estimated size of 7.23 Mbp (G+C content 45.4%). Strain CECT 9623 appeared on wet mounts as straight rods, mostly in pairs, sometimes forming long filaments (up to 20 µm). Its draft genome is shorter, with an estimated size of 6.45 Mbp (G+C content is 46.1%). Overall genome relatedness indexes clearly define them as separate organisms, so based on all the data collected, we propose the species sp. nov. with type strain AB1 (=CECT 9275=LMG 32341) and sp. nov. with type strain AB67 (=CECT 9623=LMG 32342).

摘要

本研究旨在通过对分离自饮用水系统的两株细菌 CECT 9275 和 CECT 9623 进行部分 16S rRNA 基因序列比较,更好地对其进行特征描述和分类。因此,我们在此报告对这两株菌进行的表型、基因组和系统发育特征分析。两株菌在 R2A 琼脂上生长,形成粘液状、亮黄色菌落,在 26°C 下 48 小时即可生长。它们能产生 flexirubin,氧化酶和触酶均为阳性,兼性嗜温和非嗜盐。CECT 9275 菌株的细胞为弯曲的杆状,主要以对的形式存在,形成几乎封闭的环或类似于数字 3 的形状,到长的螺旋形,类似于螺旋形。其草图基因组估计大小为 7.23 Mbp(GC 含量为 45.4%)。CECT 9623 菌株在湿载片上表现为直杆状,主要以对的形式存在,有时形成长丝(长达 20μm)。其草图基因组较短,估计大小为 6.45 Mbp(GC 含量为 46.1%)。总体基因组相关指数清楚地将它们定义为独立的生物体,因此基于收集到的所有数据,我们提出了一个新的物种,即 sp. nov.,其模式菌株为 AB1(=CECT 9275=LMG 32341)和 sp. nov.,其模式菌株为 AB67(=CECT 9623=LMG 32342)。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9af0/11573002/9b3b40a514c7/ijsem-74-06570-g001.jpg

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