State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, People's Republic of China.
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, People's Republic of China; Center of Analysis and Testing, Nanchang University, Nanchang 330047, People's Republic of China.
Food Chem. 2020 Mar 30;309:125755. doi: 10.1016/j.foodchem.2019.125755. Epub 2019 Oct 25.
A simple, sensitive and environmentally-friendly method for determining organophosphorus and pyrethroid pesticides in vegetables was developed to better evaluate the risk of consuming them. The pesticides in vegetables were extracted, purified and concentrated by using the QuEChERS (quick, easy, cheap, effective, rugged and safe method) combined DLLME-SFO (dispersive liquid-liquid microextraction based on solidification of floating organic droplet) techniques. The key parameters were optimized through orthogonal array experimental design and statistical analysis. The linearity of the calibration curves was satisfied in matrix-matched standard solution with R ≥ 0.99. The limits of detection and limits of quantification were 0.3-1.5 and 0.9-4.7 μg/kg, respectively. The average recoveries of pesticides were 61.6-119.4% with relative standard deviations <16.1%. Furthermore, the method was applied successfully to analyse the pesticides in 15 pairs of organic and conventional vegetables. These results reflect the efficiency, reliability and robustness of the developed method.
为了更好地评估食用蔬菜中有机磷和拟除虫菊酯类农药的风险，开发了一种简单、灵敏、环保的方法来检测蔬菜中的有机磷和拟除虫菊酯类农药。采用 QuEChERS（快速、简单、廉价、有效、坚固和安全方法）与 DLLME-SFO（基于漂浮有机液滴固化的分散液液微萃取）技术相结合，对蔬菜中的农药进行提取、净化和浓缩。通过正交试验设计和统计分析对关键参数进行了优化。在基质匹配标准溶液中，校准曲线的线性关系良好，R≥0.99。该方法的检出限和定量限分别为 0.3-1.5 和 0.9-4.7μg/kg。农药的平均回收率为 61.6-119.4%，相对标准偏差<16.1%。此外，该方法还成功地应用于分析 15 对有机和常规蔬菜中的农药。这些结果反映了所开发方法的效率、可靠性和稳健性。
