Liu Xinyan, Song Ru, Wei Rongbian
Key Laboratory of Health Risk Factors for Seafood of Zhejiang Province, School of Food Science and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, China.
School of Chemistry and Bioengineering, Guangxi Normal University for Nationalities, Chongzuo 532200, China.
Nanomaterials (Basel). 2022 Apr 6;12(7):1226. doi: 10.3390/nano12071226.
A method using polypyrrole-coated FeO (FeO@PPy composites) based extraction coupled with high performance liquid chromatography was developed for adsorption and detection of trace vitamin D (VD) in aquatic products. The fabricated FeO@PPy composites were characterized by scanning electron microscopy, transmission electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, and thermogravimetric analysis. FeO@PPy composites showed efficient adsorption of VD at pH 9.0 and 25 °C with a dose of 25 mg per 10 mL of sample solution and an adsorption time of 11 min. Methanol was selected as the desorption solvent to recover VD from FeO@PPy composites after 3 min of static treatment. FeO@PPy composites can be used for VD adsorption at least two times. The developed method showed a good linearity for VD determination in the range of 0.1-10 μg/mL with a correlation coefficient of 0.9989. The limits of detection and quantification were 10 ng/mL and 33 ng/mL, respectively. The recovery of VD in a spiking test was 97.72% with a relative standard deviation value of 1.78%. The content of VD in nine aquatic products was determined with this method. Our results show that FeO@PPy composites provide a convenient method for the adsorption and determination of VD from the complex matrix of aquatic products.
开发了一种基于聚吡咯包覆的FeO（FeO@PPy复合材料）萃取结合高效液相色谱的方法，用于吸附和检测水产品中的痕量维生素D（VD）。通过扫描电子显微镜、透射电子显微镜、X射线衍射、傅里叶变换红外光谱和热重分析对制备的FeO@PPy复合材料进行了表征。FeO@PPy复合材料在pH 9.0、25℃条件下，每10 mL样品溶液加入25 mg剂量、吸附时间为11 min时，对VD表现出高效吸附。选择甲醇作为解吸溶剂，在静态处理3 min后从FeO@PPy复合材料中回收VD。FeO@PPy复合材料可用于VD吸附至少两次。所开发的方法在0.1 - 10 μg/mL范围内对VD测定具有良好的线性，相关系数为0.9989。检测限和定量限分别为10 ng/mL和33 ng/mL。加标试验中VD的回收率为97.72%，相对标准偏差值为1.78%。用该方法测定了9种水产品中VD的含量。我们的结果表明，FeO@PPy复合材料为从水产品复杂基质中吸附和测定VD提供了一种便捷方法。
