National Institute of Plant Genome Research, Jawaharlal Nehru University Campus, Aruna Asaf Ali Marg, New Delhi 110067, India.
National Institute of Plant Genome Research, Jawaharlal Nehru University Campus, Aruna Asaf Ali Marg, New Delhi 110067, India.
J Proteomics. 2020 Feb 10;212:103542. doi: 10.1016/j.jprot.2019.103542. Epub 2019 Nov 5.
Chloroplast, the photosynthetic machinery, converts photoenergy to ATP and NADPH, which powers the production of carbohydrates from atmospheric CO and HO. It also serves as a major production site of multivariate pro-defense molecules, and coordinate with other organelles for cell defense. Chloroplast harbors 30-50% of total cellular proteins, out of which 80% are membrane residents and are difficult to solubilize. While proteome profiling has illuminated vast areas of biological protein space, a great deal of effort must be invested to understand the proteomic landscape of the chloroplast, which plays central role in photosynthesis, energy metabolism and stress-adaptation. Therefore, characterization of chloroplast proteome would not only provide the foundation for future investigation of expression and function of chloroplast proteins, but would open up new avenues for modulation of plant productivity through synchronizing chloroplastic key components. In this review, we summarize the progress that has been made to build new understanding of the chloroplast proteome and implications of chloroplast dynamicsing generate metabolic energy and modulating stress adaptation.
叶绿体是光合作用的细胞器，将光能转化为 ATP 和 NADPH，为大气 CO 和 HO 中的碳水化合物生产提供动力。它也是多元防御分子的主要产生部位，并与其他细胞器协同作用进行细胞防御。叶绿体含有 30-50%的总细胞蛋白，其中 80%是膜驻留蛋白，难以溶解。虽然蛋白质组学分析已经阐明了广阔的生物蛋白质空间领域，但为了理解在光合作用、能量代谢和应激适应中发挥核心作用的叶绿体的蛋白质组学景观，必须投入大量的努力。因此，叶绿体蛋白质组的特征不仅为未来研究叶绿体蛋白的表达和功能提供了基础，而且为通过同步叶绿体关键成分来调节植物生产力开辟了新途径。在这篇综述中，我们总结了在构建对叶绿体蛋白质组的新认识方面所取得的进展，以及叶绿体动态产生代谢能量和调节应激适应的意义。
