Government College of Pharmacy, Kathora Naka, Amravati, 444 604, Maharashtra State, India; Institute of Pharmaceutical Education and Research, Wardha, 442 001, Maharashtra State, India.
Government College of Pharmacy, Kathora Naka, Amravati, 444 604, Maharashtra State, India.
J Ethnopharmacol. 2020 Mar 1;249:112374. doi: 10.1016/j.jep.2019.112374. Epub 2019 Nov 5.
The aerial parts of Barleria prionitis Linn. (BP) (Acanthaceae) plant has long been used to treat inflammatory disorders such as toothache, swellings, arthritis and gout.
The purpose of this study was to evaluate the effects of shanzhiside methyl ester (SME), 8-O-acetyl shanzhiside methyl ester (ASME) and iridoid glycosides rich monoterpenoidal fraction (IFBp), isolated from the aerial part of BP, on the pro-inflammatory mediators in stimulated rat neutrophils.
Rat neutrophils were incubated with or without test drugs. The influence of laboratory isolated and identified SME, ASME and IFBp on the production and release of pro-inflammatory mediators i.e. myeloperoxidase (MPO), elastase, matrix metalloproteinase-9 (MMP-9), interleukin 8 (IL-8), tumor necrosis factor alpha (TNF-α) and leukotriene B4 (LTB4) was evaluated in the formyl-met-leu-phenylalanine (f-MLP) and lipopolysaccharide (LPS) stimulated rat neutrophils using enzyme-linked immunosorbent assay (ELISA) methods. IFBp was also standardized with the high performance thin layer chromatography by simultaneous determination of SME and ASME marker compounds.
SME, ASME and IFBp displayed concentration-dependent inhibitory effects on the MPO, elastase and MMP-9 enzymes release, and IL-8, TNF-α and LTB4 cytokines production in the f-MLP and LPS stimulated rat neutrophils. The content of SME and ASME was found to be 17.32 ± 1.98 and 11.30 ± 1.06% w/w, respectively, in IFBp by HPTLC method.
Altogether, the present results suggest that the iridoidal glycosides of BP may be considered as therapeutic strategy against neutrophil-mediated inflammatory diseases. Developed and validated HPTLC method for the standardization of IFBp of BP can be used as a quality control tool for the routine qualitative and quantitative analysis of Barleria species containing SME and/or ASME.
巴尔勒利亚·普赖恩蒂斯·林恩（BP）（爵床科）植物的地上部分长期以来一直被用于治疗炎症性疾病，如牙痛、肿胀、关节炎和痛风。
本研究旨在评估从 BP 地上部分分离得到的山栀苷甲酯（SME）、8-O-乙酰山栀苷甲酯（ASME）和富含单萜苷元的环烯醚萜糖苷部分（IFBp）对刺激大鼠中性粒细胞中促炎介质的影响。
用或不用试验药物孵育大鼠中性粒细胞。采用酶联免疫吸附试验（ELISA）方法，评价实验室分离鉴定的 SME、ASME 和 IFBp 对甲酰基-甲硫氨酸-苯丙氨酸（f-MLP）和脂多糖（LPS）刺激大鼠中性粒细胞中产生和释放促炎介质，即髓过氧化物酶（MPO）、弹性蛋白酶、基质金属蛋白酶-9（MMP-9）、白细胞介素 8（IL-8）、肿瘤坏死因子-α（TNF-α）和白三烯 B4（LTB4）的影响。IFBp 也通过同时测定 SME 和 ASME 标记化合物的高效薄层色谱法进行了标准化。
SME、ASME 和 IFBp 对 f-MLP 和 LPS 刺激的大鼠中性粒细胞中 MPO、弹性蛋白酶和 MMP-9 酶的释放以及 IL-8、TNF-α 和 LTB4 细胞因子的产生均表现出浓度依赖性抑制作用。HPTLC 法测定 IFBp 中 SME 和 ASME 的含量分别为 17.32±1.98%和 11.30±1.06%。
总之，本研究结果表明，BP 的环烯醚萜苷可能被认为是治疗中性粒细胞介导的炎症性疾病的一种治疗策略。为 BP 的 IFBp 制定的开发和验证的 HPTLC 方法可作为含有 SME 和/或 ASME 的巴尔勒利亚属植物的常规定性和定量分析的质量控制工具。
