Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, Anhui Agricultural University, Hefei, 230036, PR China.
Microb Pathog. 2020 Jan;138:103849. doi: 10.1016/j.micpath.2019.103849. Epub 2019 Nov 5.
Avian colibacillosis is one of the most serious infectious bacterial diseases that endanger the modern poultry industry. Lactobacillus is believed to inhibit intestinal pathogens and maintain a healthy gut microbiota. This study aimed to investigate Lactobacillus supplementation in Cherry Valley ducks to prevent the intestinal flora dysbiosis caused by Duck Escherichia coli 17. One hundred and twenty healthy one day old Cherry Valley ducks were randomized to three study groups (Group I = the control group; Group II = duck Escherichia coli 17 challenge group and Group III = DE17 challenge group supplemented with lactic acid bacteria composite preparation). Cherry Valley ducks in Group II and Group III were gavage challenged with DE17 (1 × 10 CFU/mL) on day 14. Pyrosequencing of the V3/V4 variable regions of the genes encoding for 16S rRNA was used for sequence analysis. The results showed that the normal intestinal microecology was affected by DE17, including a relative increase in proteobacteria. At the same time, the Lactobacillales were increased and harmful bacteria were decreased in different intestinal segments of ducks in Group III, compared to those in Group II. Network analysis showed that dietary lactic acid bacteria addition improved the interaction pattern within the cecal microbiota of ducks and the result showed that in Ruminococcus_2 was independently present in the group III and Lachnospiraceae_NK4A136_group species correlation existed between group I and group III. This study proved that oral supplementation with Lactobacillus casei 1.2435, Lactobacillus rhamnosus 621 and Lactobacillus rhamnosus A4 can mitigate DE17 induced intestinal flora dysbiosis.
禽大肠杆菌病是危害现代家禽业的最严重的传染性细菌性疾病之一。乳杆菌被认为可以抑制肠道病原体并维持健康的肠道微生物群。本研究旨在研究在樱桃谷鸭中补充乳杆菌以防止由鸭大肠杆菌 17 引起的肠道菌群失调。将 120 只 1 日龄健康的樱桃谷鸭随机分为三组研究（I 组为对照组；II 组为鸭大肠杆菌 17 攻毒组；III 组为 DE17 攻毒组补充乳酸复合制剂）。II 组和 III 组的樱桃谷鸭在第 14 天用 DE17（1×10 CFU/mL）灌胃攻毒。使用基因编码 16S rRNA 的 V3/V4 可变区的焦磷酸测序进行序列分析。结果表明，DE17 影响了正常的肠道微生态，包括变形菌门的相对增加。同时，与 II 组相比，III 组鸭的不同肠道段中乳杆菌科增加，有害细菌减少。网络分析表明，日粮添加乳酸菌改善了鸭盲肠微生物群内的相互作用模式，结果表明，在 III 组中独立存在的 Ruminococcus_2 与 I 组和 III 组之间存在 Lachnospiraceae_NK4A136_group 种相关性。本研究证明，口服补充干酪乳杆菌 1.2435、鼠李糖乳杆菌 621 和鼠李糖乳杆菌 A4 可以减轻 DE17 诱导的肠道菌群失调。
