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从相关人类供体中生成四条无整合的诱导多能干细胞系。

Generation of four integration-free iPSC lines from related human donors.

作者信息

Ramme Anja Patricia, Faust Daniel, Koenig Leopold, Marx Uwe

机构信息

TissUse GmbH, Oudenarder Str. 16, 13347 Berlin, Germany.

TissUse GmbH, Oudenarder Str. 16, 13347 Berlin, Germany.

出版信息

Stem Cell Res. 2019 Dec;41:101615. doi: 10.1016/j.scr.2019.101615. Epub 2019 Oct 19.

DOI:10.1016/j.scr.2019.101615
PMID:31704539
Abstract

Four integration-free iPSC lines were generated by reprogramming peripheral blood mononuclear cells with episomal vectors. All four human donors (two male and two female donors) belong to one Caucasian family within three different generations with the age between 19-82 years. Additionally, all iPSC lines are approved for commercial use by donor consent. Those iPSC lines offer the opportunity to study the influence of affiliation within one family. In future, more iPSCs lines of many more family members can be created to understand the effects of relatives with different ages on the reprogramming into iPSCs and differentiation into specific cell types.

摘要

通过使用游离型载体重编程外周血单核细胞，产生了四条无整合的诱导多能干细胞（iPSC）系。所有四名人类供体（两名男性供体和两名女性供体）来自一个白种人家庭的三代之内，年龄在19至82岁之间。此外，所有iPSC系均经供体同意批准用于商业用途。这些iPSC系为研究一个家庭内的亲属关系影响提供了机会。未来，可以创建更多家庭成员的更多iPSC系，以了解不同年龄的亲属对重编程为iPSC以及分化为特定细胞类型的影响。

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