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从一名健康供体获取的真皮成纤维细胞中生成人诱导多能干细胞系CBTCi001-A。

Generation of human iPS cell line CBTCi001-A from dermal fibroblasts obtained from a healthy donor.

作者信息

Martins Gabriele Louise Soares, Paredes Bruno Diaz, Sampaio Gabriela Louise de Almeida, Nonaka Carolina Kymie Vasques, da Silva Katia Nunes, Allahdadi Kyan James, França Luciana Souza de Aragão, Soares Milena Botelho Pereira, Dos Santos Ricardo Ribeiro, Souza Bruno Solano de Freitas

机构信息

Center for Biotechnology and Cell Therapy, São Rafael Hospital, Salvador, BA, Brazil; Gonçalo Moniz Institute, FIOCRUZ, Salvador, BA, Brazil.

Center for Biotechnology and Cell Therapy, São Rafael Hospital, Salvador, BA, Brazil; D'Or Institute for Research and Education (IDOR), Rio de Janeiro, RJ, Brazil.

出版信息

Stem Cell Res. 2019 Dec;41:101630. doi: 10.1016/j.scr.2019.101630. Epub 2019 Oct 21.

DOI:10.1016/j.scr.2019.101630
PMID:31706097
Abstract

Human-induced pluripotent stem cell (hiPSC) CBTCi001-A line was generated from a healthy 30-year old male dermal fibroblasts using non-integrative reprogramming method using episomal-based plasmids expressing OCT4, SOX2, KLF4, and MYCL. Characterization of CBTCi001-A was confirmed by the expression of typical markers of pluripotency and differentiation potential in vitro.

摘要

人诱导多能干细胞（hiPSC）CBTCi001-A系由一名30岁健康男性的皮肤成纤维细胞通过使用表达OCT4、SOX2、KLF4和MYCL的游离型质粒的非整合重编程方法生成。通过体外多能性和分化潜能的典型标志物的表达证实了CBTCi001-A的特性。

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