Department of Gastroenterological Surgery (Surgery II), Nagoya University Graduate School of Medicine, Nagoya, Japan.
Department of Gastroenterological Surgery (Surgery II), Nagoya University Graduate School of Medicine, Nagoya, Japan
Anticancer Res. 2019 Nov;39(11):6015-6023. doi: 10.21873/anticanres.13807.
BACKGROUND/AIM: We previously reported that expression of melanoma-associated antigen (MAGE)-D4 mRNA was a prognostic factor for esophageal squamous cell carcinoma (ESCC). The aim of this study was to validate the expression of MAGE-D4 in two additional patient cohorts, and to investigate its biological functions.
The role of MAGE-D4 in cell proliferation, adhesion, and migration was determined by gene knockdown experiments in the KYSE590 cell line. MAGE-D4 protein expression was analyzed in ESCC tissues by immunohistochemistry. A second validation cohort consisted of an ESCC mRNA dataset from The Cancer Genome Atlas.
Knockdown of MAGE-D4 significantly decreased cell proliferation and migration. Expression of MAGE-D4 protein was significantly associated with disease-free survival. In the second validation cohort, high MAGE-D4 mRNA expression was associated with significantly shorter overall survival and disease-free survival.
MAGE-D4 plays an important role in the malignant behavior of ESCC. MAGE-D4 was validated as a prognostic indicator in two independent ESCC patient cohorts.
背景/目的：我们之前报道过黑色素瘤相关抗原（MAGE）-D4mRNA 的表达是食管鳞状细胞癌（ESCC）的预后因素。本研究的目的是在另外两个患者队列中验证 MAGE-D4 的表达，并研究其生物学功能。
通过在 KYSE590 细胞系中进行基因敲低实验，确定 MAGE-D4 在细胞增殖、黏附和迁移中的作用。通过免疫组织化学分析 ESCC 组织中的 MAGE-D4 蛋白表达。第二个验证队列由癌症基因组图谱（TCGA）的 ESCCmRNA 数据集组成。
MAGE-D4 的敲低显著降低了细胞增殖和迁移。MAGE-D4 蛋白的表达与无病生存期显著相关。在第二个验证队列中，高 MAGE-D4mRNA 表达与总生存期和无病生存期显著缩短相关。
MAGE-D4 在 ESCC 的恶性行为中起重要作用。MAGE-D4 在两个独立的 ESCC 患者队列中被验证为预后指标。
