LRG1 在食管鳞癌中的临床预后价值。

The Clinical Prognostic Value of LRG1 in Esophageal Squamous Cell Carcinoma.

机构信息

Department of Radiation Oncology, Qilu Hospital of Shandong University, Jinan, Shandong, China.

Department of Oncology, Linyi People's Hospital, Dezhou, Shandong, China.

出版信息

Curr Cancer Drug Targets. 2019;19(9):756-763. doi: 10.2174/1568009619666190204095942.

DOI:10.2174/1568009619666190204095942

PMID:30714525

Abstract

BACKGROUND

Leucine-rich-alpha-2-glycoprotein1 (LRG1) is a new oncogene-related gene, which has been proven important for the development and poor prognosis of human cancers. However, whether it participates in esophageal squamous cell carcinoma (ESCC) progression remains unclear.

OBJECTIVE

To investigate the expression level and functional influence of LRG1 in ESCC.

METHODS

The expression of LRG1 was evaluated on the mRNA and protein level in ESCC patients. Then, correlation of LRG1 expression with clinicpathological variables was analyzed in ESCC. Besides, to clarify the biological function of LRG1, Eca109 and KYSE150 cells were transfected with LRG1 shRNA, the cell viability, clonal efficiency, apoptosis and invasion assays in vitro were performed.

RESULTS

LRG1 was significantly over-expressed in ESCC and related to deeper invasion depth (T stage) and distal metastasis (M stage). Kaplan-Meier analysis indicated that LRG1 up-regulation in ESCC was closely correlated to worse clinical survival (overall survival and progression-free survival), all P<0.001. LRG1 was confirmed to be an independent poor premonitory indicator for clinical outcomes in ESCC through the univariate and multivariate analyses. Down-regulation of LRG1 in ESCC cells markedly suppressed cell proliferation and invasion, stimulated apoptosis (all p <0.01).

CONCLUSION

LRG1 might play a significant role in the progression of ESCC, and could be served as a promising prognostic prediction for ESCC patients.

摘要

背景

富含亮氨酸α-2-糖蛋白 1（LRG1）是一种新的癌基因相关基因，已被证明对人类癌症的发展和预后不良至关重要。然而，它是否参与食管鳞状细胞癌（ESCC）的进展尚不清楚。

目的

研究 LRG1 在 ESCC 中的表达水平和功能影响。

方法

在 ESCC 患者中评估 LRG1 的 mRNA 和蛋白水平表达。然后，分析 LRG1 表达与 ESCC 临床病理变量的相关性。此外，为了阐明 LRG1 的生物学功能，用 LRG1 shRNA 转染 Eca109 和 KYSE150 细胞，进行体外细胞活力、克隆效率、凋亡和侵袭测定。

结果

LRG1 在 ESCC 中显著过表达，并与更深的浸润深度（T 分期）和远处转移（M 分期）相关。Kaplan-Meier 分析表明，ESCC 中 LRG1 的上调与更差的临床生存（总生存和无进展生存）密切相关，均 P<0.001。通过单因素和多因素分析，LRG1 被证实是 ESCC 临床结局的独立不良预后指标。在 ESCC 细胞中下调 LRG1 可显著抑制细胞增殖和侵袭，刺激凋亡（均 p <0.01）。