Department of Anesthesiology and Pain Medicine, Chungnam National University Sejong Hospital, Sejong 30099, Korea.
Department of Anesthesiology and Pain Medicine, Chungnam National University College of Medicine, Daejeon 35015, Korea.
Medicina (Kaunas). 2021 Jan 7;57(1):45. doi: 10.3390/medicina57010045.
There are several studies that sevoflurane could enhance proliferation of cancer cells, while others suggest no effect on clinical outcome. We conducted in vivo and in vitro experiments to investigate the effects of sevoflurane, a volatile anesthetic, on proliferation and outcomes of Lewis lung carcinoma (LLC) cells. A total of 37 mice were injected with LLC cells to compare the tumor size and survival of the sevoflurane exposed group (sevo group) and control group. The sevo group was exposed to 2% sevoflurane and 4 L/min of oxygen for 1 h per day 3 times per week, and the control group was exposed only to 4 L/min of oxygen. In vitro study, 12 plates incubated with LCC cells. 6 plates were exposed to 2% sevoflurane for 1 hr/day for 3 days and 6 plates were not exposed, and cell proliferation was compared after 3 days. There were no significant differences in survival or tumor size between mice exposed to sevoflurane and control mice (survival: 29.06 ± 4.45 vs. 28.76 ± 3.75, = 0.836; tumor size: 0.75 (0.41-1.02) vs. 0.49 (0.11-0.79), = 0.153). However, in vitro study, the proliferation of LLC cells exposed to sevoflurane increased by 9.2% compared to the control group ( = 0.018). Sevoflurane (2 vol%) exposure could promote proliferation of LLC cells in vitro environment, but may not affect proliferation of LLC cells in vivo environment. These results suggest that in vitro studies on the effects of anesthetics on cancer may differ from those of in vivo or clinical studies.
有几项研究表明，七氟醚可以促进癌细胞的增殖，而其他研究则表明其对临床结果没有影响。我们进行了体内和体外实验，以研究挥发性麻醉剂七氟醚对 Lewis 肺癌（LLC）细胞增殖和结果的影响。共有 37 只小鼠注射 LLC 细胞，比较暴露于七氟醚的实验组（sev 组）和对照组的肿瘤大小和存活情况。sev 组每天暴露于 2%七氟醚和 4 L/min 氧气中 1 小时，每周 3 次，而对照组仅暴露于 4 L/min 氧气中。体外研究中，有 12 个培养板孵育 LLC 细胞。其中 6 个板每天暴露于 2%七氟醚中 1 小时，连续 3 天，而另外 6 个板不暴露，比较 3 天后细胞增殖情况。暴露于七氟醚的小鼠与对照组小鼠的存活或肿瘤大小无显著差异（存活：29.06±4.45 对 28.76±3.75， = 0.836；肿瘤大小：0.75（0.41-1.02）对 0.49（0.11-0.79）， = 0.153）。然而，体外研究表明，暴露于七氟醚的 LLC 细胞的增殖比对照组增加了 9.2%（ = 0.018）。七氟醚（2 体积%）暴露可促进 LLC 细胞在体外环境中的增殖，但可能不会影响体内环境中 LLC 细胞的增殖。这些结果表明，麻醉剂对癌症影响的体外研究可能与体内或临床研究不同。
