Sikora Bartosz, Skubis-Sikora Aleksandra, Kimsa-Furdzik Małgorzata, Ciszek Wojciech, Kostrzewski Marek, Stojko Jerzy, Mazurek Urszula, Gola Joanna
Department of Cytophysiology, Chair of Histology and Embryology, Faculty of Medical Sciences in Katowice, Medical University of Silesia, Katowice, Poland.
Department of Biochemistry, Faculty of Medical Sciences in Katowice, Medical University of Silesia, Katowice, Poland.
Stem Cell Res. 2019 Dec;41:101609. doi: 10.1016/j.scr.2019.101609. Epub 2019 Oct 24.
Mesenchymal stem cells (MSCs) are objects of interest in regenerative medicine. They are used for various therapies such as for the regeneration of bone, chondrocytes and other tissues. Adipose derived stem cells (ADSCs) inter alia are particularly easy to access, they are relatively abundant in fat tissue. ADSCs could be differentiated into many types of cells. To date, it has been proven that ADSCs only differentiate into mesodermal cell lineages. In this study, we present the differentiation of ADSCs into the corneal epithelium. Human ADSCs were placed in a co-culture with porcine limbal epithelial stem cells (LESCs). After 14 days of cultivation, total RNA was extracted for the analysis of the molecular markers (expression of genes of interest). The gene expression was assessed by real-time RT-qPCR. The expression of the surface molecular markers of ADSCs is modulated after co-culturing. We have observed the decrease in CD73, CD90 and CD105 mRNA expression, while the expression of mRNA coding for CK3 and CK12 mRNA was increased in ADSCs co-cultured with porcine limbal epithelial stem cells as compared to the control. We conclude that the co-culture of LESCs and ADSCs changed ADSCs' molecular markers gene expression indicating initiation of differentiation towards limbal cells.
间充质干细胞（MSCs）是再生医学领域的研究热点。它们被用于多种治疗，如骨、软骨细胞及其他组织的再生。脂肪来源干细胞（ADSCs）尤其易于获取，在脂肪组织中含量相对丰富。ADSCs可分化为多种类型的细胞。迄今为止，已证实ADSCs仅能分化为中胚层细胞谱系。在本研究中，我们展示了ADSCs向角膜上皮细胞的分化。将人ADSCs与猪角膜缘上皮干细胞（LESCs）共培养。培养14天后，提取总RNA用于分析分子标志物（感兴趣基因的表达）。通过实时RT-qPCR评估基因表达。共培养后，ADSCs表面分子标志物的表达受到调节。我们观察到，与对照组相比，在与猪角膜缘上皮干细胞共培养的ADSCs中，CD73、CD90和CD105 mRNA表达下降，而编码CK3和CK12 mRNA的mRNA表达增加。我们得出结论，LESCs与ADSCs的共培养改变了ADSCs分子标志物的基因表达，表明其开始向角膜缘细胞分化。
