College of Life Science, Luoyang Normal University, Luoyang, 471934, China.
Huaiyin Institute of Agricultural Sciences of Xuhuai Region in Jiangsu, Huaian, 223001, China.
Ecotoxicol Environ Saf. 2020 Jan 30;188:109894. doi: 10.1016/j.ecoenv.2019.109894. Epub 2019 Nov 6.
Modulation of plant salt tolerance has been drawing great attention. Thymol is a kind of natural chemical that has been developed as anti-microbial reagent and medicine. To date, we still have limited knowledge about thymol-modulated plant physiology. In this work, physiological, histochemical, and biochemical methods were adopted to study thymol-conferred salt resistance in the root of rice (Oryza sativa). Thymol significantly rescued root growth under salt stress. Thymol ameliorated cell membrane damage, oxidative stress, ROS accumulation, and cell death in roots under salt stress. Thymol-attenuated oxidative stress may be resulted from the activation of anti-oxidative capacity, including both enzymatic and non-enzymatic system. Thymol treatment significantly decreased Na content in root cells upon salt stress, which might be ascribed to the upregulation of OsSOS1 (salt overly sensitive 1) facilitating Na exclusion. In addition, thymol stimulated the expression of genes encoding tonoplast OsNHX (Na/Hantiporter), which may help root cells to compartmentalize Na in vacuole. The results of these works evidenced that thymol was capable of inducing salt tolerance by reestablishing ROS homeostasis and modulating cellular Na flux in rice roots. These findings may be applicable to improve crop growth in salinity area.
植物耐盐性的调控一直受到广泛关注。百里香酚是一种天然化学物质，已被开发为抗菌试剂和药物。迄今为止，我们对百里香酚调节植物生理学的了解仍然有限。在这项工作中，采用生理、组织化学和生化方法研究了百里香酚赋予水稻（Oryza sativa）根的耐盐性。百里香酚在盐胁迫下显著挽救了根的生长。百里香酚改善了盐胁迫下根中的细胞膜损伤、氧化应激、ROS 积累和细胞死亡。百里香酚减轻的氧化应激可能是由于抗氧化能力的激活，包括酶和非酶系统。百里香酚处理在盐胁迫下显著降低了根细胞中的 Na 含量，这可能归因于 OsSOS1（盐过度敏感 1）的上调促进了 Na 外排。此外，百里香酚刺激了液泡膜 Na/Hantiporter 基因的表达，这有助于根细胞将 Na 区室化到液泡中。这些研究结果表明，百里香酚能够通过重新建立 ROS 平衡和调节水稻根中的细胞内 Na 流来诱导耐盐性。这些发现可能适用于改善盐渍地区的作物生长。
