Cameron I L, Contreras E, Fullerton G D, Kellermayer M, Ludány A, Miseta A
Department of Cellular and Structural Biology, University of Texas Health Science Center, San Antonio 78284.
J Cell Physiol. 1988 Oct;137(1):125-32. doi: 10.1002/jcp.1041370115.
Crystalline lenses provided good material to study and measure the properties of cellular water. Different methods were used to establish the extent and properties of nonbulk water in mammalian lenses. These methods include: NMR titration analysis, a test of the osmotic properties, a test of dye exclusion In lenses with intact cell membranes and in lenses with disrupted cell membranes, and the water-holding capacity of lenses subjected to 40,000 x g for 1 hour with intact cell membranes and in lenses with disrupted cell membranes. The data from these methods, as well as other data from the literature, lead to the conclusion that most, if not all, of the water in lens cells (up to 2.2 g water/g dry mass) has motional and osmotic properties that distinguish it from bulk water. These findings call into question the common and convenient assumption that all but a small proportion of cellular water is like that in dilute solution.
晶状体为研究和测量细胞内水的性质提供了良好的材料。人们使用了不同的方法来确定哺乳动物晶状体中非大量水的范围和性质。这些方法包括：核磁共振滴定分析、渗透压性质测试、完整细胞膜晶状体和破损细胞膜晶状体的染料排斥测试，以及完整细胞膜晶状体和破损细胞膜晶状体在40,000×g离心1小时后的持水能力测试。这些方法获得的数据以及文献中的其他数据得出这样的结论：晶状体细胞中大部分（如果不是全部的话）水（高达每克干重2.2克水）具有与大量水不同的运动和渗透性质。这些发现对一个普遍且便捷的假设提出了质疑，即除一小部分外，所有细胞内的水都与稀溶液中的水相似。
