McChrisley B, Thye F W, McNair H M, Driskell J A
Department of Human Nutrition and Foods, Virginia Polytechnic Institute and State University, Blacksburg 24061.
J Chromatogr. 1988 Jun 24;428(1):35-42. doi: 10.1016/s0378-4347(00)83888-8.
A rapid and sensitive procedure is described for the analysis of all the B6 vitamers and 4-pyridoxic acid in human plasma utilizing reversed-phase high-performance liquid chromatography with ultraviolet and fluorometric detection. Pyridoxal phosphate values obtained by radiometric and chromatographic, ultraviolet and fluorometric, assays were highly correlated as were pyridoxine phosphate values determined using both detectors. Plasma B6 vitamer and 4-pyridoxic acid concentrations of 22 men fed diets containing 0.75-0.98 mg of vitamin B6 daily for eight weeks were in the range of reported values; pyridoxal phosphate was their predominant plasma B6 vitamer. This methodology should be useful in the assessment of vitamin B6 requirements.
本文描述了一种快速灵敏的方法，用于利用反相高效液相色谱结合紫外和荧光检测分析人血浆中的所有维生素B6异构体和4-吡哆酸。通过放射性测定法、色谱法、紫外和荧光测定法获得的磷酸吡哆醛值高度相关，使用两种检测器测定的磷酸吡哆醇值也是如此。22名男性连续八周每日摄入含0.75 - 0.98毫克维生素B6的饮食，其血浆维生素B6异构体和4-吡哆酸浓度在报告值范围内；磷酸吡哆醛是其主要的血浆维生素B6异构体。该方法在评估维生素B6需求量方面应具有实用性。
