National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA; National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD, USA.
Trends Mol Med. 2020 Jan;26(1):58-70. doi: 10.1016/j.molmed.2019.09.009. Epub 2019 Nov 6.
The specific cellular role of mitochondria is influenced by the surrounding environment because effective mitochondrial function requires the delivery of inputs (e.g., oxygen) and export of products (e.g., signaling molecules) to and from other cellular components, respectively. Recent technological developments in mitochondrial imaging have led to a more precise and comprehensive understanding of the spatial relationships governing the function of this complex organelle, opening a new era of mitochondrial research. Here, I highlight current imaging approaches for visualizing mitochondrial form and function within complex cellular environments. Increasing clarity of mitochondrial behavior within cells will continue to lend mechanistic insights into the role of mitochondria under normal and pathological conditions and point to spatially regulated processes that can be targeted to improve cellular function.
线粒体的具体细胞作用受到周围环境的影响，因为有效的线粒体功能分别需要向其他细胞成分输入（例如氧气）和输出产物（例如信号分子）。线粒体成像技术的最新发展使我们能够更精确和全面地了解控制这个复杂细胞器功能的空间关系，开辟了线粒体研究的新时代。在这里，我重点介绍了当前用于在复杂细胞环境中可视化线粒体形态和功能的成像方法。线粒体在细胞内行为的清晰度不断提高，将继续为线粒体在正常和病理条件下的作用提供机制上的见解，并指出可以针对空间调节过程来改善细胞功能。
