College of Environmental Science and Engineering, Taiyuan University of Technology, Taiyuan, 030024, Shanxi, People's Republic of China.
College of Mining Engineering, Taiyuan University of Technology, Taiyuan, 030024, Shanxi, People's Republic of China.
World J Microbiol Biotechnol. 2019 Nov 9;35(11):179. doi: 10.1007/s11274-019-2760-4.
High concentration restaurant oily wastewater from restaurants and food processing industries discharged into water environment usually results in environment pollution and inhibits the activity of microorganisms in biological wastewater treatment systems. In this study, 75 strains from oily sludge were isolated with oil degradation activity for edible oil-contained wastewater. Eight isolates were able to grow well in liquid cultures with edible oil as the sole carbon source and discovered with high efficient oil-degrading ability. Seven out of eight isolates were identified as Acinetobacter and one isolate as Kluyvera cryocrescens, based on their 16S rRNA gene sequences. Three highly efficient oil degrading bacteria (Acinetobacter dijkshoorniae LYC46-2, Kluyvera cryocrescens LYC50-1a and Acinetobacter pittii LYC73-4b) were selected and their degradation characteristic were examined, the results showed that the three isolates were effective under pH range from 7.0 to 10.0, and temperature from 25 to 35 °C. For degradation of 2-4% (v/v) of vegetable oil, > 85% degradation percentage were obtained within 30 h. Degradation of the higher concentration oil (6-8%, v/v) result in 50-70% degradation percentage within 72 h, and the degradation percentage for the isolated strains were decreased about 50% for the degradation of 10% oil (< 45%) compared to 2% oil. Different type of oils were also tested, > 90% of degradation percentage were obtained by the three isolates, implied that these strains are capable of removing various oils efficiently. These results suggested that Acinetobacter dijkshoorniae LYC46-2, Kluyvera cryocrescens LYC50-1a and Acinetobacter pittii LYC73-4b are potential species could be efficiently used for high concentration restaurant oily wastewater treatment and might be applicable to a wastewater treatment system for the removal of oil.
从餐馆和食品加工行业排放到水环境中的高浓度餐饮含油废水通常会导致环境污染，并抑制生物废水处理系统中的微生物活性。在这项研究中，从含油污泥中分离出 75 株具有降解食用油能力的菌株，用于含食用油废水。有 8 株菌能够在以食用油为唯一碳源的液体培养基中良好生长，并发现具有高效的油脂降解能力。根据 16S rRNA 基因序列，这 8 株菌中有 7 株被鉴定为不动杆菌，1 株被鉴定为克氏克鲁维酵母。选择了 3 株高效的油脂降解菌（不动杆菌 dijkshoorniae LYC46-2、克氏克鲁维酵母 LYC50-1a 和不动杆菌 pittii LYC73-4b），并对其降解特性进行了研究，结果表明，这 3 株菌在 pH 值为 7.0-10.0 和温度为 25-35°C 的范围内有效。在降解 2-4%（v/v）的植物油时，在 30 h 内获得了>85%的降解率。在 72 h 内，降解更高浓度的油（6-8%，v/v）导致 50-70%的降解率，与降解 2%的油相比，降解率下降了约 50%（<45%）。还测试了不同类型的油，这 3 株菌的降解率均>90%，这表明这些菌株能够有效地去除各种油。这些结果表明，不动杆菌 dijkshoorniae LYC46-2、克氏克鲁维酵母 LYC50-1a 和不动杆菌 pittii LYC73-4b 是具有高效去除高浓度餐饮含油废水的潜在物种，可能适用于去除油的废水处理系统。
