Matsumiya Yoshiki, Wakita Daisuke, Kimura Akishige, Sanpa Sirilak, Kubo Motoki
Department of Bioscience and Technology, Faculty of Science and Engineering, Ritsumeikan University, Nojihigashi, Kusatsu, Shiga 525-8577, Japan.
J Biosci Bioeng. 2007 Apr;103(4):325-30. doi: 10.1263/jbb.103.325.
To construct an efficient lipid-containing wastewater treatment system, microorganisms that degrade lipids efficiently were isolated from various environmental sources. Strain DW2-1 showed the highest rate of degradation of 1% (w/v) salad oil among the isolated strains. Strain DW2-1 was identified as Burkholderia sp. and designated Burkholderia sp. DW2-1. The rate of degradation of salad oil, olive oil, sesame oil, and beef tallow by strain DW2-1 were 96.7%, 92.3%, 90.1% and 77.4%, respectively, during a 48-h cultivation. Strain DW2-1 grew well in a synthetic wastewater medium (>1 x 10(10) colony forming unit [CFU]/ml) between 20 degrees C and 38 degrees C, and its rate of degradation of salad oil was above 90% after a 48-h cultivation. The lipase and biosurfactant (BSF) activities of strain DW2-1 after a 48-h cultivation were 1720 U/l and 480 U/ml, respectively. In continuous cultures for lipid-containing wastewater treatment, DW2-1 was stably maintained and degraded more than 90% of salad oil during a 7-d cultivation.
为构建高效的含油废水处理系统，从各种环境源中分离出了能高效降解油脂的微生物。在分离出的菌株中，菌株DW2-1对1%（w/v）色拉油的降解率最高。菌株DW2-1被鉴定为伯克霍尔德菌属，并命名为伯克霍尔德菌属DW2-1。在48小时的培养过程中，菌株DW2-1对色拉油、橄榄油、芝麻油和牛脂的降解率分别为96.7%、92.3%、90.1%和77.4%。菌株DW2-1在20℃至38℃的合成废水培养基中生长良好（>1×10¹⁰菌落形成单位[CFU]/ml），48小时培养后其对色拉油的降解率高于90%。菌株DW2-1在48小时培养后的脂肪酶和生物表面活性剂（BSF）活性分别为1720 U/l和480 U/ml。在用于含油废水处理的连续培养中，DW2-1在7天的培养过程中稳定存在，对色拉油的降解率超过90%。
