Brantley R K, Bass A H
Section of Neurobiology and Behavior, Cornell University, Ithaca, New York 14853.
J Comp Neurol. 1988 Sep 1;275(1):87-105. doi: 10.1002/cne.902750108.
A monoclonal antibody (Ab8) to choline acetyltransferase (ChAT) was used to locate structures showing ChAT-like immunoreactivity (ChAT-IR) in the brain of a teleost fish, the midshipman (Porichthys notatus). ChAT is the synthetic enzyme for acetylcholine found in neurons using that neurotransmitter; thus ChAT-IR may be interpreted as indicating putative cholinergic activity. Robust staining is seen in all cranial nerve motor nuclei. In addition, the brainstem of Porichthys is distinguished by two other expansive ChAT-IR zones: a sonic motor nucleus, which innervates swimbladder "drum" muscles, and an octavolateralis efferent nucleus, which innervates acoustic, vestibular, and lateral line end organs. Scattered labeled cells are found in several cranial sensory nuclei--the vagal lobe, and the main and descending trigeminal nuclei. ChAT-IR cells form restricted subpopulations in other noncranial nerve nuclei, including the granule cell layer of the cerebellum; superior, medial, and inferior divisions of the reticular formation; the stratum periventriculare of the midbrain's optic tectum; and the nucleus isthmi in the midbrain tegmentum. In the telencephalon, a dense population of ChAT-IR cells is found in the ventral nucleus of area ventralis; terminals and fine fibers are found in the dorsal, medial, and central nuclei of area dorsalis. Together, the data represent the first complete report of ChAT-IR cell bodies in the brain of any nonmammal with the monoclonal antibody Ab8, which has already been extensively used on a variety of vertebrate brains. The results are thus discussed from a comparative viewpoint, considering reports of ChAT-IR in different taxa.
一种针对胆碱乙酰转移酶（ChAT）的单克隆抗体（Ab8）被用于定位硬骨鱼——海鲈鱼（Porichthys notatus）脑中显示ChAT样免疫反应性（ChAT-IR）的结构。ChAT是在使用该神经递质的神经元中发现的乙酰胆碱合成酶；因此，ChAT-IR可被解释为指示假定的胆碱能活性。在所有脑神经运动核中均可见到强烈的染色。此外，海鲈鱼的脑干还有另外两个广泛的ChAT-IR区：一个是支配鱼鳔“鼓”肌的发声运动核，另一个是支配听觉、前庭和侧线终器的八侧线传出核。在几个脑神经感觉核——迷走叶、三叉神经主核和降核中发现了散在的标记细胞。ChAT-IR细胞在其他非脑神经核中形成受限的亚群，包括小脑颗粒细胞层；网状结构的上、中、下部分；中脑视顶盖的室周层；以及中脑被盖的峡核。在端脑中，腹侧区腹侧核中有密集的ChAT-IR细胞群；在背侧区的背核、内侧核和中央核中发现了终末和细纤维。这些数据共同代表了使用单克隆抗体Ab8对任何非哺乳动物脑内ChAT-IR细胞体的首次完整报告，该抗体已在多种脊椎动物脑上广泛使用。因此，本文从比较的角度，结合不同分类群中ChAT-IR的报告对结果进行了讨论。
