Xia Mengfan, Sui Yucun, Guo Ying, Zhang Yaodong
Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710119, China.
Analyst. 2021 Feb 8;146(3):904-910. doi: 10.1039/d0an02076a.
A fluorescence analysis method based on gold nanocluster (AuNC) and metal-organic framework (MOF) composite materials (AuNCs@ZIF-8) was established for highly sensitive detection of bilirubin (BR). First, AuNCs@ZIF-8 was successfully obtained by co-precipitation and displayed an aggregation-induced emission enhancement by the confinement effect of the MOFs (i.e., ZIF-8). The product showed approximately 7.0 times enhancement in the quantum yield and longer fluorescence lifetime from 2.29 μs to 11.51 μs compared with AuNCs. When BR combined with the metal node Zn2+ of ZIF-8, the skeleton of the composite was destroyed, leading to a great decrease in the fluorescence intensity by the transformation of the AuNCs from the aggregated state to dispersed state. The linear range for the detection of BR was 0.1-5.0 μM, with the limit of detection (LOD) of 0.07 μM (S/N = 3). The AuNCs@ZIF-8 exhibited a selective response toward BR within 5 min and detected BR in human serum. The long-wavelength emission by AuNCs avoided the interference of the complex biomatrix background fluorescence, indicating their great application prospects for clinical diagnosis.
建立了一种基于金纳米簇（AuNC）和金属有机框架（MOF）复合材料（AuNCs@ZIF-8）的荧光分析方法，用于高灵敏度检测胆红素（BR）。首先，通过共沉淀法成功获得了AuNCs@ZIF-8，并且由于MOF（即ZIF-8）的限域效应，其表现出聚集诱导发光增强。与AuNCs相比，该产物的量子产率提高了约7.0倍，荧光寿命从2.29 μs延长至11.51 μs。当BR与ZIF-8的金属节点Zn2+结合时，复合材料的骨架被破坏，导致AuNCs从聚集态转变为分散态，荧光强度大幅下降。BR的检测线性范围为0.1 - 5.0 μM，检测限（LOD）为0.07 μM（S/N = 3）。AuNCs@ZIF-8在5分钟内对BR表现出选择性响应，并能够检测人血清中的BR。AuNCs的长波长发射避免了复杂生物基质背景荧光的干扰，表明其在临床诊断中具有广阔的应用前景。
