Henan Key Laboratory of Rare Earth Functional Materials, International Joint Research Laboratory for Biomedical Nanomaterials of Henan, Zhoukou Normal University, Zhoukou 466001, China.
Anal Methods. 2022 Apr 7;14(14):1439-1444. doi: 10.1039/d2ay00195k.
Gold nanoclusters (AuNCs) have large Stokes shifts and long fluorescence life, which make them have high application value in bioanalytical applications. However, the quantum yield (QY) of AuNCs was lower, which hinders their wide application. Herein, a facile, novel and one-pot approach was developed to synthesize AuNCs@zinc-glutamate metal organic frameworks (AuNCs@ZnGlu-MOFs (product)). The product was easily prepared mixing the glutathione (GSH) protected AuNCs with ZnGlu-MOF precursors. Compared with GSH-AuNCs, the ultrahigh QY (33.18%) of the AuNCs@ZnGlu-MOF is nearly 6 times higher. In addition, the product possesses better water stability and longer luminescence life (9.86 μs) due to the protective and confinement effects of the ZnGlu-MOF. Particularly, the product has a unique spatial structure, which can effectively prevent the interaction between large-size biothiols (such as cysteine and homocysteine) and the product, thus significantly improving the selectivity of it. Based on the excellent optical advantages of this product, it was capable of being applied as a selectable and sensitive fluorescence probe to detect HO and HO-related analytes. This method has also been further employed in the precise HO-monitoring in serum, which is promising in the application of clinical bioassay.
金纳米团簇 (AuNCs) 具有较大的斯托克斯位移和较长的荧光寿命，这使得它们在生物分析应用中具有很高的应用价值。然而，AuNCs 的量子产率 (QY) 较低，这阻碍了它们的广泛应用。本文开发了一种简便、新颖的一锅法合成金纳米团簇@谷氨酸锌金属有机框架 (AuNCs@ZnGlu-MOFs（产物）)。该产物是通过将谷胱甘肽 (GSH) 保护的 AuNCs 与 ZnGlu-MOF 前体混合而容易制备的。与 GSH-AuNCs 相比，AuNCs@ZnGlu-MOF 的超高 QY（33.18%）几乎提高了 6 倍。此外，由于 ZnGlu-MOF 的保护和限制作用，产物具有更好的水分稳定性和更长的荧光寿命（9.86 μs）。特别是，该产物具有独特的空间结构，可有效防止大尺寸生物硫醇（如半胱氨酸和同型半胱氨酸）与产物之间的相互作用，从而显著提高其选择性。基于该产物优异的光学优势，它可用作选择性和灵敏的荧光探针来检测 HO 和 HO 相关分析物。该方法还进一步用于血清中精确的 HO 监测，在临床生物测定应用中具有广阔的前景。
