Firestone A R, Graves C N, Feagin F F
Department of Restorative Dentistry, School of Dentistry, Univeristy of Alabama, Birmingham 35294.
J Dent Res. 1988 Oct;67(10):1342-5. doi: 10.1177/00220345880670101901.
Three groups of weanling, Sprague-Dawley-derived rats were inoculated with Actinomyces viscosus M-100 and fed powdered diet containing either 67%, 5%, or 0% confectioner's sugar. Two further groups were fed diet containing 5% confectioner's sugar and inoculated with Streptococcus sobrinus 6715 or S. sobrinus 6715 plus A. viscosus M-100. The most coronal 1 mm of gingiva was removed from maxillary and mandibular right molar quadrants (gingivectomy), and the animals re-inoculated following gingivectomy. The animals were killed 64 days following gingivectomy, and the lingual surface of mandibular first molar roots was measured for exposed root-surface area and root caries. In the groups of rats infected with A. viscosus M-100, root caries area was significantly greater in the group fed diet containing 67% confectioner's sugar. Sucrose level did not significantly affect the amount of exposed lingual first molar root area regardless of whether the tooth had been subjected to a gingivectomy. In the groups of rats receiving diet containing 5% confectioner's sugar, there were no significant differences in root caries area or exposed root-surface area, regardless of the infection status of the animals. In the rat model presented here, a high level of dietary sucrose was a necessary condition for the initiation of root caries in the absence of other readily fermentable carbohydrates.
将三组断奶的斯普拉格-道利大鼠接种粘性放线菌M-100，并喂食含有67%、5%或0%细砂糖的粉状饲料。另外两组喂食含有5%细砂糖的饲料，并接种远缘链球菌6715或远缘链球菌6715加粘性放线菌M-100。从大鼠上颌和下颌右侧磨牙象限切除最靠近牙冠的1毫米牙龈（牙龈切除术），并在牙龈切除术后对动物重新接种。牙龈切除术后64天处死动物，测量下颌第一磨牙牙根舌面的暴露根面面积和根龋情况。在感染粘性放线菌M-100的大鼠组中，喂食含有67%细砂糖饲料的组根龋面积明显更大。无论牙齿是否接受过牙龈切除术，蔗糖水平对下颌第一磨牙舌侧暴露根面面积的影响均不显著。在喂食含有5%细砂糖饲料的大鼠组中，无论动物的感染状态如何，根龋面积或暴露根面面积均无显著差异。在此处呈现的大鼠模型中，在没有其他易发酵碳水化合物的情况下，高糖饮食是引发根龋的必要条件。
