Department of Agricultura, Ramaderia, Pesca, Caça i Cooperació Municipal, Consell Insular d'Eivissa, Balearic Islands, Spain.
Research Group on Community Nutrition and Oxidative Stress, IUNICS, University of Balearic Islands, Balearic Islands, Spain.
J Fish Dis. 2020 Jan;43(1):1-7. doi: 10.1111/jfd.13085. Epub 2019 Nov 10.
Perkinsus mediterraneus is a protozoan parasite that can cause marine mollusc diseases known as perkinsosis being a serious threat for clam cultures worldwide. The aim of the present study was first to determine the Perkinsus species infecting the variegated scallop Mimachlamys varia and then to evaluate the existence of oxidative stress in gills of M. varia according to different degrees of infection. DNA sequencing confirmed that P. mediterraneus was the species infecting M. varia. ROS production was progressively increasing with the degree of infection although the differences were only significant in the high-infected group. Low degree of infection significantly increased superoxide dismutase (SOD) and glutathione S-transferase (GST) activities and nitrite levels with respect to the control group. In the high-infected group, a significant increase was evidenced in all analysed enzymes, catalase, SOD, glutathione reductase and GST. Non-significant differences in MDA levels were observed between the control and low-infected groups; however, a significant increase in MDA levels was observed in the high-infected group. In conclusion, the infection by Perkinsus mediterraneus in M. varia induces oxidative stress and an antioxidant response directly related to the infection degree that can contribute to the pathogenicity of the infection.
地中海巴贝斯虫是一种原生动物寄生虫，可引起海洋软体动物疾病，称为帕氏单孢子虫病，对全球蛤类养殖构成严重威胁。本研究的目的首先是确定感染杂色蛤 Mimachlamys varia 的巴贝斯虫种类，然后根据不同感染程度评估杂色蛤 M. varia 鳃中的氧化应激情况。DNA 测序证实感染 M. varia 的物种为地中海巴贝斯虫。尽管在高感染组中差异仅具有统计学意义，但随着感染程度的增加，ROS 的产生逐渐增加。低度感染与对照组相比，超氧化物歧化酶（SOD）和谷胱甘肽 S-转移酶（GST）活性以及亚硝酸盐水平显著升高。在高感染组中，所有分析的酶（过氧化氢酶、SOD、谷胱甘肽还原酶和 GST）活性均显著增加。对照组和低度感染组之间 MDA 水平无显著差异；然而，在高感染组中 MDA 水平显著升高。综上所述，地中海巴贝斯虫感染杂色蛤 M. varia 会诱导氧化应激和抗氧化反应，这与感染程度直接相关，可能有助于感染的致病性。
