食高胆固醇、高脂肪饮食的食蟹猴长期受到挑战后早期动脉粥样硬化病变的组织学变化。
Histological variation of early stage atherosclerotic lesions in baboons after prolonged challenge with high-cholesterol, high-fat diet.
机构信息
Department of Internal Medicine, Wake Forest University Health Sciences, Winston-Salem, NC, USA.
Department of Genetics, Texas Biomedical Research Institute, San Antonio, TX, USA.
出版信息
J Med Primatol. 2020 Feb;49(1):3-9. doi: 10.1111/jmp.12449. Epub 2019 Nov 10.
INTRODUCTION
The baboon is a well-characterized model of human early stage atherosclerosis. However, histological and morphological changes involved in atherogenesis in baboons are not known. Previously, we challenged baboons with a high-cholesterol, high-fat diet for two years and observed fatty streak and plaque lesions in iliac arteries (RCIA).
METHODS
We evaluated histological and morphological changes of baboon arterial lesions and control arteries. In addition, we evaluated the vascular expression of CD68 and SMαA markers with progression of atherosclerosis.
RESULTS
We observed changes that correlated with extent of atherosclerosis, including increased maximum intimal thickness. We demonstrated at molecular level the infiltration of smooth muscle cells and macrophages into the intimal layer. Further, we observed histological and morphological discordancy between the affected and adjacent areas of the same RCIA.
CONCLUSION
Atherogenesis in baboons is accompanied by histological, morphological, and molecular changes, as in humans, providing insights to evaluate the mechanisms underlying early stage atherosclerosis in target tissues.
简介
狒狒是研究人类早期动脉粥样硬化的一种特征明确的模型。然而，关于狒狒动脉粥样硬化形成过程中的组织学和形态学变化尚不清楚。此前，我们用高胆固醇、高脂肪饮食喂养狒狒两年，观察到其髂动脉（RCIA）出现了脂肪条纹和斑块病变。
方法
我们评估了狒狒动脉病变和对照动脉的组织学和形态学变化。此外，我们还评估了动脉粥样硬化进展过程中 CD68 和 SMαA 标志物的血管表达情况。
结果
我们观察到与动脉粥样硬化程度相关的变化，包括内膜最大厚度增加。我们在分子水平上证明了平滑肌细胞和巨噬细胞浸润到内膜层。此外，我们还观察到同一 RCIA 的受影响区域和相邻区域之间存在组织学和形态学差异。
结论
与人类一样，狒狒的动脉粥样硬化形成伴随着组织学、形态学和分子变化，为评估靶组织中早期动脉粥样硬化的机制提供了思路。
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